The Cardinal Bernardin Cancer Center is located on the east side of the campus and faces First Avenue. Parking is available in a parking lot in front of the building and valet parking is available at the entrance. A coffee bar is located just inside the building on the first floor.

Named in honor of the late Archbishop of Chicago Joseph Cardinal Bernardin, the cancer center was the first free-standing facility in Illinois dedicated to cancer research, diagnosis, treatment and prevention. Loyola's cancer center contains all outpatient cancer care along with extensive research laboratories, offices and educational space.

Many of the multidisciplinary clinics within the cancer center provide a one-visit, one-team approach, providing patients with a diagnosis and treatment plan in the same day. Patients can see their physician, have lab work done, undergo chemotherapy and have cancer care-related prescriptions filled, among many other services in the building.