Multi-Tracer Pet Quantitation of Insulin Action
| Status: | Completed |
|---|---|
| Conditions: | Diabetes |
| Therapuetic Areas: | Endocrinology |
| Healthy: | No |
| Age Range: | 30 - 55 |
| Updated: | 4/2/2016 |
| Start Date: | July 2007 |
| End Date: | June 2010 |
| Contact: | Nicole Helbling, RN, MS |
| Email: | nlr8@pitt.edu |
| Phone: | 412-692-2285 |
We are proposing a clinical investigation of the pathogenesis of insulin resistance (IR) in
skeletal muscle and adipose tissue (AT), focusing specifically on the contributions of
glucose delivery, transport and phosphorylation. The primary methodology will be dynamic PET
imaging, using three tracers that respectively portray the kinetics of glucose delivery,
bi-directional trans-membrane glucose transport and glucose phosphorylation. The three
tracers are: 1) [15O]-H2O for quantifying tissue perfusion, this portrays the kinetics of
glucose delivery from plasma to tissue; 2) [11C]-3-O-methyl glucose, a tracer constrained to
bi-directional trans-membrane glucose transport; and 3) [18F]-fluoro-deoxy glucose, which
like [11C]-3-OMG is transported, but adds the subsequent metabolic step, that of glucose
phosphorylation.
We propose 2 specific aims to apply this methodology to investigate the pathogenesis of IR.
The 1st aim is to quantitatively assess the kinetics of glucose delivery, transport and
phosphorylation in skeletal muscle in type 2 DM and as compared to obese and lean
non-diabetic men and women. We will appraise the contribution of each step to the to the
pathogenesis of IR. We postulate more severe IR in oxidative muscle, with a dual impairment
of glucose transport and phosphorylation. The 2nd aim is to implement the triple-tracer
dynamic PET imaging protocol in adipose tissue (AT), examining normal insulin action in
non-obese volunteers and testing whether differences in AT insulin action are present in
obese insulin sensitive volunteers compared to obese IR participants and the relation of AT
IR to that of muscle and liver.
skeletal muscle and adipose tissue (AT), focusing specifically on the contributions of
glucose delivery, transport and phosphorylation. The primary methodology will be dynamic PET
imaging, using three tracers that respectively portray the kinetics of glucose delivery,
bi-directional trans-membrane glucose transport and glucose phosphorylation. The three
tracers are: 1) [15O]-H2O for quantifying tissue perfusion, this portrays the kinetics of
glucose delivery from plasma to tissue; 2) [11C]-3-O-methyl glucose, a tracer constrained to
bi-directional trans-membrane glucose transport; and 3) [18F]-fluoro-deoxy glucose, which
like [11C]-3-OMG is transported, but adds the subsequent metabolic step, that of glucose
phosphorylation.
We propose 2 specific aims to apply this methodology to investigate the pathogenesis of IR.
The 1st aim is to quantitatively assess the kinetics of glucose delivery, transport and
phosphorylation in skeletal muscle in type 2 DM and as compared to obese and lean
non-diabetic men and women. We will appraise the contribution of each step to the to the
pathogenesis of IR. We postulate more severe IR in oxidative muscle, with a dual impairment
of glucose transport and phosphorylation. The 2nd aim is to implement the triple-tracer
dynamic PET imaging protocol in adipose tissue (AT), examining normal insulin action in
non-obese volunteers and testing whether differences in AT insulin action are present in
obese insulin sensitive volunteers compared to obese IR participants and the relation of AT
IR to that of muscle and liver.
We propose a clinical investigation of the pathogenesis of insulin resistance (IR) in
skeletal muscle and adipose tissue (AT) in obesity and diabetes mellitus, focusing on the
separate and interactive roles of glucose delivery, bi-directional trans-membrane glucose
transport and glucose phosphorylation. The primary methodology will be dynamic PET imaging,
using three tracers that respectively portray the kinetics of glucose delivery, transport
and phosphorylation. The three tracers are: 1) [15O]-H2O for quantifying tissue perfusion,
this parameter together with glucose concentration portrays the kinetics of glucose delivery
from plasma to tissue interstitial space; 2) [11C]-3-O-methyl glucose, a tracer constrained
to bi-directional trans-membrane glucose transport; and 3) [18F]-fluoro-deoxy glucose, which
like [11C]-3-OMG is transported, but adds the subsequent metabolic step, that of glucose
phosphorylation.
In our recently completed studies, we implemented this triple-tracer dynamic PET imaging
protocol to investigate insulin action in lean, healthy individuals 1-3. Rates of glucose
uptake can be obtained by other methods (e.g. the glucose clamp, arterio-venous limb
balance). What is uniquely valuable with dynamic PET imaging is acquisition of a temporal
plot of tracer uptake, one that is obtained within an organ rather than derived from plasma
determinations. These tissue-time activity curves provide information to assess the velocity
of metabolic steps. By doing this for each of the three tracers, assessment can be made of
which among glucose delivery, transport and phosphorylation is rate-controlling, or more
properly, how rate control is distributed amongst these steps. The triple-tracer procedure
has provided novel, quantitative insight on the action of insulin to change this
distribution of control, a re-distribution triggered in healthy individuals by robust
activation of glucose transport. Robust activation of glucose transport increases
permeability of muscle to glucose sufficiently that delivery manifests greater rate
limitation than during basal conditions. Also, we have coupled PET bio-imaging with MRI to
study specific muscles 1, 3. This approach has yielded provocative and unanticipated new
findings. Unlike in lean non-diabetics, in whom oxidative muscle is more insulin sensitive
(as widely demonstrated in animal studies), in type 2 and in type 1 DM, oxidative muscle is
more severely IR. We are encouraged that this bio-imaging methodology will enable new
insight into the pathogenesis of IR in skeletal muscle and that the approach can be
successfully adapted for in vivo investigation of adipose tissue metabolism.
The 1st specific aim is to quantitatively assess the contribution of glucose delivery,
transport and phosphorylation to the pathogenesis of skeletal muscle IR in type 2 DM and
obesity.
The 2nd specific aim is to implement triple-tracer dynamic PET imaging to study insulin
action in gluteal-femoral adipose tissue (GF-AT) of non-obese and obese women, investigating
among the latter group mechanisms of IR of GF-AT, and the role that GF-AT IR may have in
differentiating obese insulin-sensitive (OB-InS) from obese insulin-resistant (OB-IR) women.
Experiment Synopsis: During the past year, in pilot studies, we initiated PET imaging
procedures for AT, using [18F]-FDG. We now propose full development of the triple tracer
methodology in GF-AT. Non-obese and obese women will be studied, the latter recruited to
form groups of obese insulin-sensitive (OB-IS) and obese insulin-resistant (OB-IR).
Triple-tracer PET imaging will be done during basal and insulin stimulated conditions, using
an infusion rate of 20 mU/min-m2. Complementary assessments will include: a) MRI and DXA to
measure the quantity of fat-mass (FM), GF-AT, abdominal adipose depots (ABD-SAT and VAT); b)
endogenous glucose production (EGP) assessed using a primed, constant infusion of [6,6]
d2-glucose; c) an adipokine profile; and d) a needle biopsy of GF-AT for histological and
other analyses.
skeletal muscle and adipose tissue (AT) in obesity and diabetes mellitus, focusing on the
separate and interactive roles of glucose delivery, bi-directional trans-membrane glucose
transport and glucose phosphorylation. The primary methodology will be dynamic PET imaging,
using three tracers that respectively portray the kinetics of glucose delivery, transport
and phosphorylation. The three tracers are: 1) [15O]-H2O for quantifying tissue perfusion,
this parameter together with glucose concentration portrays the kinetics of glucose delivery
from plasma to tissue interstitial space; 2) [11C]-3-O-methyl glucose, a tracer constrained
to bi-directional trans-membrane glucose transport; and 3) [18F]-fluoro-deoxy glucose, which
like [11C]-3-OMG is transported, but adds the subsequent metabolic step, that of glucose
phosphorylation.
In our recently completed studies, we implemented this triple-tracer dynamic PET imaging
protocol to investigate insulin action in lean, healthy individuals 1-3. Rates of glucose
uptake can be obtained by other methods (e.g. the glucose clamp, arterio-venous limb
balance). What is uniquely valuable with dynamic PET imaging is acquisition of a temporal
plot of tracer uptake, one that is obtained within an organ rather than derived from plasma
determinations. These tissue-time activity curves provide information to assess the velocity
of metabolic steps. By doing this for each of the three tracers, assessment can be made of
which among glucose delivery, transport and phosphorylation is rate-controlling, or more
properly, how rate control is distributed amongst these steps. The triple-tracer procedure
has provided novel, quantitative insight on the action of insulin to change this
distribution of control, a re-distribution triggered in healthy individuals by robust
activation of glucose transport. Robust activation of glucose transport increases
permeability of muscle to glucose sufficiently that delivery manifests greater rate
limitation than during basal conditions. Also, we have coupled PET bio-imaging with MRI to
study specific muscles 1, 3. This approach has yielded provocative and unanticipated new
findings. Unlike in lean non-diabetics, in whom oxidative muscle is more insulin sensitive
(as widely demonstrated in animal studies), in type 2 and in type 1 DM, oxidative muscle is
more severely IR. We are encouraged that this bio-imaging methodology will enable new
insight into the pathogenesis of IR in skeletal muscle and that the approach can be
successfully adapted for in vivo investigation of adipose tissue metabolism.
The 1st specific aim is to quantitatively assess the contribution of glucose delivery,
transport and phosphorylation to the pathogenesis of skeletal muscle IR in type 2 DM and
obesity.
The 2nd specific aim is to implement triple-tracer dynamic PET imaging to study insulin
action in gluteal-femoral adipose tissue (GF-AT) of non-obese and obese women, investigating
among the latter group mechanisms of IR of GF-AT, and the role that GF-AT IR may have in
differentiating obese insulin-sensitive (OB-InS) from obese insulin-resistant (OB-IR) women.
Experiment Synopsis: During the past year, in pilot studies, we initiated PET imaging
procedures for AT, using [18F]-FDG. We now propose full development of the triple tracer
methodology in GF-AT. Non-obese and obese women will be studied, the latter recruited to
form groups of obese insulin-sensitive (OB-IS) and obese insulin-resistant (OB-IR).
Triple-tracer PET imaging will be done during basal and insulin stimulated conditions, using
an infusion rate of 20 mU/min-m2. Complementary assessments will include: a) MRI and DXA to
measure the quantity of fat-mass (FM), GF-AT, abdominal adipose depots (ABD-SAT and VAT); b)
endogenous glucose production (EGP) assessed using a primed, constant infusion of [6,6]
d2-glucose; c) an adipokine profile; and d) a needle biopsy of GF-AT for histological and
other analyses.
Inclusion Criteria:
Male and Female Normal Weight - non-diabetic (BMI 19-25) Overweight/Obese - non-diabetic
(BMI 27-38) Type 2 DM (BMI 27-38)
Fasting lab glucose < 100 mg/dl (non-diabetic groups) HbA1c < 6.0 (non-diabetic group)
HbA1c < 8.5 (diabetic group)
Ulnar artery patent bilaterally Negative urine pregnancy test Non-smoker Independent in
self blood glucose monitoring (diabetic group)
Exclusion Criteria:
BP > 150 mmHg systolic or > 95 mmHg diastolic History of any heart disease, including MI,
pacemaker History of PVD, (including diminishing pulses) liver disease, kidney disease,
pulmonary disease, neuromuscular disease, neurological disease, thyroid disease or any
drug or alcohol abuse.
Current malignancy or history of cancer within the past 5 years Proteinuria 1+ or greater
Hematocrit < 34% sTSH >8 ALT > 60; AST > 60; Alk Phos > 150 Total cholesterol > 250
Triglycerides > 300
MEDICATIONS:
Chronic medications that can alter glucose homeostasis: oral glucocorticoids, nicotinic
acid (Birth control medications are okay and will not exclude) Thiazolidinediones or
insulin, previous difficulty with lidocaine (xylocaine) Gained or lost more than 3 kg
during the past 3 months Involved in regular exercise > 1 day/week Surgical or vascular
implants, any metal in body, claustrophic Currently pregnant OR currently lactating
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