Intracoronary Bradykinin Mediated t-PA Release in Heart Transplant Recipients

Inclusion criteria:

1. Heart transplant recipients undergoing annual cardiac catheterization who have
participated our protocol: Characterization of brachial arterial t-PA release,
vasodilator function, and vascular compliance and correlation with fibrinolytic
balance, oxidative stress, and inflammation measures in heart transplant recipients
(SCCOR Project 1, Aim 3C). (IRB # 070517)

2. 25 Subjects will have transplant vasculopathy and 25 subjects will be free of
transplant vasculopathy, as documented in previous angiograms.

3. Otherwise healthy

Exclusion criteria:

1. PVC < 30

2. Hypertensive subjects on ACE inhibitors

3. Pregnant or nursing mothers

4. Diabetic with HbA1C > 7.5 or stigmata of end organ damage (neuropathy, retinopathy,
nephropathy, cardiomyopathy)

5. Cholesterol > 30 mg/dL above NCEP accepted level based on cardiac risk.

6. Triglycerides > 200

7. Previously diagnosed obstructive coronary artery disease

8. Renal insufficiency (Creatinine ≥ 1.5 mg/dl)

9. History of cerebrovascular disease

10. Any chronic inflammatory disease (rheumatologic, inflammatory bowel disease, etc)

11. Uncontrolled Stage 2 Hypertension (160/100 mmHg), or end organ damage due to
hypertension (left ventricular hypertrophy, atrial fibrillation, hematuria, renal
insufficiency, prior cerebrovascular disease).

12. Angiotensin converting enzyme inhibitor use

13. Coagulopathy (INR ≥ 1.5, PTT ≥ 150% of control)

14. Peripheral Vascular Disease

15. Other chronic medical illnesses at the discretion of the investigators

Healthy controls are being enrolled in SCCOR Project 1, Aims 3A and 3B (IRB# 030473 and
061160) and will not be participating under this IRB number.