Reversal of Tobacco-Related Sinusitis

By using patient screening questionnaires and measuring hair nicotine, a well acknowledged
biomarker of exposure to tobacco smoke, we will attempt a more objective study to examine
the association between tobacco smoke and chronic rhinosinusitis. (CRS) Likewise, zinc
deficiency is documented in numerous animal and human studies to decrease resistance to
infectious diseases and is especially common among smokers. We will explore our hypothesis
that SHS exposure and zinc deficiency contribute to CRS. To accomplish this, we will
measure serum zinc and hair nicotine levels in CRS patients and correlate them to a variety
of CRS diagnostic indicators. A second objective of this study is investigating alterations
within the epithelium lining the sinonasal cavities in patients with chronic rhinosinusitis.
The alterations may be one or a combination of anatomic, genetic, inflammatory, or
infectious etiologies. To further investigate these possibilities we plan on taking
residual clinical material from endoscopic sinus surgery specimens and performing various in
vitro investigations including but not limited to microarray analysis, northern and western
blot analysis, ciliary beat frequency analysis, immunohistochemistry, electron microscopy,
and propagation of tissue with tissue culture techniques. Tissues taken from non-sinusitis
patients undergoing sinonasal surgery will serve as a non-chronic rhinosinusitis control
source of sinus mucosa.

Inclusion Criteria:

- CRS and skull base surgery patients; CRS patients that meet the objective and
subjective guidelines for CRS set forth by the Sinus and Allergy Health Partnership.

Exclusion Criteria:

- Ciliary dysfunction, autoimmune disease, CF or any known immunodeficiency.