The Assessment of Oxidative Stress Markers and the Development of Atrial Fibrillation After Cardiac Surgery

Atrial Fibrillation (AF) is a common occurrence after cardiac surgery. This arrhythmia is
reported to occur in up to 40% of patients following coronary bypass graft (CABG) surgery.
The incidence of AF is even higher in patients who have undergone both CABG and valvular
surgery during the same operation. In cardiac surgery patients, the occurrence of atrial
fibrillation post-operatively lengthens hospitalization and adds to postoperative
complications like renal dysfunction, infection, and cognitive defects. Postoperative AF is
a known major risk factor for the development of stroke after cardiac surgery.
Additionally, there is data to suggest that the development of AF after cardiac surgery is
related to both reduced survival after CABG, and this dysrhythmia may also serve as an
independent predictor of long-term mortality. Many studies have investigated the role of
prophylactic pharmacological therapy to prevent post-operative AF after cardiac surgery.
Despite this, the complete pathogenesis of AF in the post-cardiac surgery population remains
unknown, is likely to be multifactorial, and includes clinical, structural, and biochemical
risk factors.

Biochemical risk factors such as inflammation and elevated markers of oxidative stress seem
to be related to the development of AF. Also, It has been shown that AF is associated with
decreased activity of nitric oxide synthases. Elevated C-reactive protein (CRP), a common
marker of inflammation, has been shown to be associated with chronic AF at one year follow
up. Additionally, elevated CRP levels drawn just prior to electrical cardioversion have been
found to predict the development of recurrent AF after cardioversion. This relationship
between this elevated marker of inflammation - CRP - and AF has been found to be independent
of clinical risk factors for the development of AF, such as age, diabetes, and left
ventricular function. Inflammation is a known source of oxidative stress at the cellular
level; and it has been proposed that both inflammation and oxidative stress play a role in
electrical remodeling of the atria, which promotes perpetuation of AF. Moreover, it has
been proposed that CRP levels may directly reflect the condition of increased oxidative
stress.

Oxidative Stress and Arrhythmia:

The association between AF and arrhythmias has been suggested by an array of evidence.
Moreover, the pathogenesis likely involves both changes in the atrial architecture, such as
dilation and fibrosis, and alterations in the electrophysiological substrate of atrial
tissue. Oxidative stress markers are believed to be involved in remodeling of the atrial
electrophysiological substrate.

Recently, a correlation between AF and oxidative stress markers has been established. In
one study, a correlation between the oxidative stress markers reduced glutathione, cysteine,
and derivatives of reduced oxidative metabolites has been found. Although the relationship
between AF and oxidative stress remains unclear, it is known from this human population
study that oxidative stress markers are elevated in those with persistent AF. This
relationship holds true even after controlling for conditions that promote oxidative stress:
hypertension, smoking, and congestive heart failure. It has also been shown that atrial
tachycardias are associated with increased hydroxyl radicals and peroxynitrite. These
species are believed to cause myofibril damage and reduced myofibrillar creatine kinase
activity in those with chronic AF. Similarly, superoxide free radicals were shown to be
elevated in the porcine heart left atrial appendage when subjected to rapid atrial pacing
and AF. Of further interest in this study by Dudley et al., was the role of an NADPH
oxidase inhibitor, apocynin, in its ability to reduce superoxide production. This suggests
that the NADPH oxidase plays a prominent role in the development of reactive oxidative
species. In turn, such reactive oxidative species are thought to be related to inflammation
and atrial electrical remodeling.

It is believed that electrical remodeling leads to changes such as shortening of the
effective refractory period (ERP), loss of rate adaptation of the refractory period, and
prolonged atrial conduction. Rapid atrial conduction likely results in increased calcium
(Ca2+) accumulation. This causes decreased inward L-type Ca2+ current (ICa), resulting in a
shorter action potential duration (APD). Additional cellular mechanisms may include
inactivation of the fast sodium current (INa) at more positive membrane potentials, and
increased inward potassium rectifier (IK1) and acetylcholine dependent potassium currents
(IKAch). Though these currents logically oppose one another, the net effect is that of
increased repolarization heterogenicity and shortened APD. Moreover, experimental models
have shown that rapid atrial pacing leads to decreased ERP and reduced of L-type Ca2+
channel and transient outward K+ ion currents. Shortening of the ERP leads to decreased
conduction wavelengths, and shortened wavelengths have been proposed to increase the
propensity for the development of AF because they reduce the mass of atrial tissue required
to sustain reentry circuits.

Given the changes above, it has been postulated that intracellular calcium overload as a
result of AF is a trigger for electrical remodeling in AF. Nevertheless, further studies
have demonstrated that blockade of L-type calcium channels alone with verapamil in canine
hearts is not sufficient to prevent atrial electrical remodeling. Additional triggering
mechanisms may include oxidative stress and inflammation. Simvastatin, a drug that has both
anti-oxidant and anti-inflammatory properties has been shown to attenuate the development of
AF from tachycardia induced atrial remodeling in dogs. More specific evidence of ion
channel remodeling from free radicals was proved by Caouette et al. who showed that addition
of hydrogen peroxide directly delayed ultra-rapid rectifier potassium currents in the
Chinese Hamster Ovary cell line. These changes are mediated by Kv1.5 gene expression, which
accounts for voltage-gated potassium repolarization currents that are similarly expressed in
human atrial and ventricular myocytes. Further ion channel studies have demonstrated that
lipid peroxidation causes Na+ channel dysfunction in cultured atrial myocytes when exposed
to the oxidizing agent tert-butyl-hydroperoxide . Additionally, it is believed that
reactive oxidative species may promote respiratory chain dysfunction in atrial myocytes by
way of mitochondrial DNA damage.

In summary, the relationship between oxidative stress and atrial fibrillation is complex and
incompletely understood on the molecular level. Nevertheless, it is clear that this
relationship does exist, and therefore serves as a potential focus for medical therapy. In
fact, Carnes et al. clearly demonstrated that using ascorbate perioperatively to blunt the
effects of oxidative stress results in both decreased peroxynitrite formation and a lower
incidence of AF after CABG. Therefore, investigators propose to do more work in this arena
to better elucidate the relationship of oxidative stress and AF.

Oxidative Stress Markers in the Research Setting:

Cardiac surgery leads to high oxidative stress and inflammation and is associated with
postoperative AF. This is the ideal situation in which to better define oxidative stress
markers and their correlation with AF. There are several ways to measure oxidative stress
in humans. A minimally invasive method is the measurement of lipid peroxides (derivatives
of reactive oxygen metabolites, dROMs) and oxidized and reduced thiol ratios. Glutathione is
an important water-phase antioxidant and essential cofactor for antioxidant enzymes. It
provides protection endogenous oxygen radicals. Since glutathione can exist in oxidized and
reduced forms, it can serve as a buffer for increased oxidative stress. Moreover, the
relative amounts of these two forms are a reflection of the oxidative state of humans. This
balance can be measured out of blood. Measurement of this ratio has been validated by Neuman
et al. and is known to be associated with AF. Additionally, these measurements have been
validated in states that are thought to increase oxidative stress, such as diabetes, older
age, and cigarette smoking.

Inclusion Criteria:

- Age ≥18 years

- Sinus rhythm at the time of enrollment as documented by ECG, telemetry, or Holter
monitoring

- Scheduled for an open heart surgery

- Able to provide informed consent

Exclusion Criteria:

- Systemic inflammatory diseases (e.g., collagen vascular diseases, infections)

- Malignant neoplasms

- Hyperthyroidism

- Uncontrolled hypertension (blood pressure > 180/100 at rest) on medications

- An illness that might result in death within 1 year

- Implanted devices for designed for active management of atrial arrhythmias by pacing
or defibrillation

- Current illicit drug use

- Current ethanol abuse

- Presence of paroxysmal, persistent, or permanent atrial fibrillation at the time of
enrollment