MAPP Investigation of Pelvic Floor-Brain Neurobiologic Axis in IC/IBS and IBS

Inclusion Criteria:Irritable Bowel Syndrome (IBS) Study Population

Inclusion criteria:

1. During the previous year, all patients must have recurrent abdominal discomfort or
pain for at least 3 days per month in the last 3 months associated with two or more of
the following symptoms (6): i) improvement with defecation; ii) onset associated with
a change in frequency of stool; and/or iii) onset associated with a change in form
(appearance) of stool (83);

2. No evidence for structural diseases (excluded by colonoscopy/ barium enema and
metabolic problem by lab tests; and

3. On a prospective symptom/stool diary [Appendix 1] patients reported i) presence of
abdominal pain/discomfort for at least 2 days per week; ii) hard or lumpy stools >25%
and loose or watery stools in < 25% of bowel movements (IBS-C); (iii) loose or watery
stools in >25% of bowel movements and hard stools <25% of BMs(IBS-D); >25% of hard or
loose stools within one week (IBS-M) (6).

Exclusion Criteria:

1. Patients with laxative abuse, anorexia nervosa, and bulimia;

2. Patients taking constipating drugs, (e.g. opioids), tricyclics (because of increased
seizure risk), serotonin modulators (tegaserod), antispasmodics (dicyclomine or
hyoscyamine), muscle relaxants (e.g. cyclobenzaprine) unless the drug is stopped at
least 2 weeks before enrollment;

3. Patients with a current history of depression and/or taking antidepressants;

4. Patients with comorbid illnesses, severe cardiac disease, chronic renal failure or
previous gastrointestinal surgery except cholecystectomy and appendectomy;

5. Patients with neurologic diseases (e.g. head injuries, epilepsy, multiple sclerosis,
strokes, spinal cord injuries) or brain disorders prone to causing seizures;

6. Patients experiencing impaired cognizance (mini mental score of < 15) and/or legally
blind;

7. Women who are pregnant or likely to conceive (women with potential for pregnancy must
use contraceptive measures to be included);

8. Patients with ulcerative and Crohns colitis;

9. Patients with rectal prolapse, anal fissure, anal surgery or inflamed hemorrhoids.

Interstitial Cystitis/Painful Bladder Syndrome (IC/PBS) Study Population

Inclusion Criteria:

IC/PBS patients who have met all three of the modified NIDDK criteria (84) for IC/PBS as
described below on a urinary symptom assessment form (Appendix 3):

1. Participants will report the occurrence of bladder pain with a score of >3 on a Likert
scale of 1-10.

2. Participants will report the presence of increased urinary frequency with a score of
>3 on a Likert scale of 1 -10.

3. The bladder pain and increased urinary frequency will have been present for at least 6
weeks.

We recognize that the NIDDK is currently working on recommending new diagnostic criteria
for interstitial cystitis. We will adopt any revised criteria commiserate with the new
recommendations of the NIDDK as our study progresses.

Exclusion Criteria:

1. History of genitourinary tuberculosis, bladder cancer, high grade dysplasia, carcinoma
in situ, urethra, vaginal, or cervical cancer.

2. Previous treatment with cytoxan or cyclophosphamide (as reported by the patient).

3. Radiation cystitis (as reported by the patient).

4. Neurogenic bladder dysfunction (e.g.,) due to spinal cord injury, stroke, Parkinson's,
MS, spina bifida, or diabetic cystopathy (reported by the patient or determined during
a neurologic exam.

5. Having had an augmentation cystoplasty (as reported by the patient).

6. Having undergone a cystectomy, cystolysis, or neuroectomy as reported by the patient.

7. Having a urethral stricture of less than 12 French (as reported by the patient).

8. Urinalysis with >10 white blood cells per high-powered field.

9. A positive urine culture for bacterial cystitis within the past three months (by
report) or a positive dipstick for leucocyte esterase or nitrates on urinary dipstick
test at the time of presentation.

10. Symptoms of vaginitis (as reported by the patient).

11. Active herpes at presentation or has had active herpes in the last three months (by
report).

12. Use of antimicrobials in the past three months for urinary tract infections (by
report).

13. Presence of bladder, ureteral, or urethral calculi (as reported by the patient).

14. Having undergone a cystometrogram, bladder cystoscopy under anesthesia or bladder
biopsy under anesthesia or urethral dilation within the 6 weeks.

15. Pregnancy.

Additional Criteria for recruitment:

Patients will be assessed no sooner than 6 weeks following a DMSO or other treatment or a
hydrodistention as these treatments may influence inflammation. Subjects should have had no
infectious illness within 2 weeks of participation. As estrogen levels are thought to
contribute to IC symptoms, and symptom exacerbation has been reported perimenstrually (85),
all menstruating subjects will be tested during the mid-luteal portions of their menstrual
cycle (approx. days 7-14) to control for hormonal variability.

We weighed the issue of excluding IC patients taking pain and psychotropic medication vs.
including these patients. Amitriptyline is commonly used for treatment of pain in both IC
and IBS; these patients commonly take other psychotropic drugs, systemic antihistamines,
and drugs for pain. If we were to exclude all patients on any medication, we would be able
to recruit a small and unrepresentative sample of the healthiest IC patients. If we asked
patients to refrain from using medication for 2 weeks before the study, we would be
subjecting them to undue pain and hardship, and might trigger an exacerbation of the
disease. However, use of antihistamines affect measurements of methylhistamine, and use of
amitriptyline and other anti- depressants produce a general blunting of the HPA response to
a stressful stimuli (86, 87) as well as modulate the neurobiologic responses. We will
exclude all subjects on antidepressants to examine a more homogeneous population. To try to
obtain as representative a sample as possible without confounding of results we used the
following strategy in our pilot studies and plan to use the same strategy in the proposed
study. Subjects taking systemic antihistamines could not be included if they had taken
antihistamines within a week of the study. Our preliminary data indicated that there was no
significant difference in the ACTH or cortisol response to the reactivity challenge among
patients using vs. those not using amitriptyline or other psychotropic medications (p's =
.45 to .90).

Healthy Controls study population:

Additionally, we will recruit 30 healthy controls matched for our patient group with
regards to age, gender, hormonal status and parity. Controls will be healthy women with no
history of urological disease or genito-urinary symptoms and no immunomodulatory disease
such as rheumatoid arthritis, lupus, MS, chronic fatigue, fibromyalgia, diabetes, cancer,
HIV, vestibulitis, allergies, chronic fungal infections, migraines, depression, or chronic
pain conditions. Healthy controls will be asked to complete the Mayo Health Screening
survey and this will be used to adjudicate their health status.

Healthy controls will be on NO meds other than multivitamins, oral contraceptives, and
possibly low dose aspirin. They can not be taking anti-hypertensives, serotonin reuptake
inhibitors, thyroid meds or other medications.