Investigations Regarding B-cell Subsets in Humans

The specific aim of this research project is to define the human equivalent of mouse B1
cells. This will be done by evaluating IgM secretion, surface markers, functional
characteristics, V gene usage, N-region addition, and phosphorylation status of
intracellular proteins, gene expression, sequencing of immunoglobulin genes, determination
of antigenic specificity, replication history, and response to different stimuli.

The goal of this project is to begin to the translation of decades of basic science research
and research in the mouse model into the human system. It is believed that properly defining
this B cell subset in humans will lead to better therapeutics, prognostics and prevention
for a number of disease including infections, autoimmune disease, and cancer.

Inclusion Criteria:

- Subject is considered by the physician to be a healthy adult.

Exclusion Criteria:

- Subject is under the age of 18

- Subject has an infection or is currently ill