Study of HF10 in Patients With Refractory Head and Neck Cancer or Solid Tumors With Cutaneous and/or Superficial Lesions

This is an open label, non-randomized, multicenter, two-stage, dose escalation Phase I study
evaluating single and repeated intratumoral injections of the oncolytic virus, HF10, in
patients with refractory head and neck cancer, or solid tumors with cutaneous and/or
superficial lesions (e.g., squamous cell carcinoma of the skin, carcinoma of the breast, and
malignant melanoma).

Stage 1: Stage 1 of the study will investigate dose escalation of a single intratumoral
injection over the following dose levels: 1 x 10^5 TCID50, 3 x 10^5 TCID50, 1 x 10^6 TCID50,
and 1 x 10^7 TCID50. In Stage 1, it is planned that 3 patients will be enrolled per single
dose cohort. Within each single dose cohort, accrual will temporarily be suspended after the
first patient is entered and the patient will be followed for safety and for viral
distribution and elimination. The patients in Stage 1 must be seropositive for HSV-1.

Stage 2: Stage 2 will evaluate repeated intratumoral injections of HF10 at dose levels of 1
x 10^6 TCID50/dose and 1 x 10^7 TCID50/dose. Three patients will be enrolled in each of the
repeated dose cohorts. In Stage 2, the first patient treated in each repeated dose cohort
must be seropositive for HSV-1. Patients in the repeated dose cohort will receive a total of
four intratumoral injections in the same lesion.

Following completion of dosing in the repeated dose cohorts, an expansion cohort of three
additional patients will be treated at the highest tolerated dose level.

Inclusion Criteria

- Patients must have histologically confirmed solid tumors that have failed standard
therapies (surgery, chemotherapy, radiotherapy, or endocrine therapy) and for which
no curative options exist, including, but not limited to:

- Squamous cell carcinoma of the head and neck

- Squamous cell carcinoma of the skin

- Carcinoma of the breast

- Malignant melanoma

- Patients may have had any kind and number of prior cancer therapies.

- Patients must have measurable non-visceral lesions that are evaluable by the RECIST
method

- The tumor mass to be treated must be non-visceral and adequate for injection (i.e.,
more than 2 cm away from major vascular structures) and measurement by RECIST.

- Patients in Stage 1 must be seropositive for HSV-1.

- The first patient enrolled into each cohort in Stage 2 must be seropositive for
HSV-1.

- Patients must be ≥ 18 years of age.

- Patients must have a life expectancy ≥ 12 weeks

- Patients must have an Eastern Cooperative Oncology Group (ECOG) performance status of
0, 1, or 2.

- Patients must have adequate hepatic function, as defined as

- Total bilirubin levels ≤ 1.5 x upper limit of normal (ULN)

- AST/ALT levels ≤ 2.5 x ULN, or ≤ 5 x ULN if liver metastases are present

- Patients must have adequate renal function as defined as serum creatinine ≤ 1.5 x ULN
or creatinine clearance (calculated) ≥ 60 mL/min/1.73 m2 for patients with creatinine
> 1.5 x ULN

- Patients must have adequate bone marrow function, as defined as

- Absolute neutrophil count ≥ 1,500/μL and

- Platelet count ≥ 100,000/μL

- Patients must have no known bleeding diathesis or coagulopathy that would make
intratumoral injection or biopsy unsafe.

- Men and women of childbearing potential must agree to use adequate contraception
prior to study entry and for up to six months.

- Females of childbearing potential must have a negative urine or serum pregnancy test
within one week prior to start of treatment.

- Patients must be able to understand and willing to sign a written informed consent
document.

Exclusion Criteria

- Patients receiving chemotherapy or radiotherapy within 4 weeks of injection of HF10,
or adverse events > Grade 1, except alopecia, resulting from agents administered more
than 4 weeks prior to HF10 injection.

- Patients with a history of significant tumor bleeding, or coagulation or bleeding
disorders.

- Patients with nasopharyngeal tumors.

- Patients with deep (below the platysma muscle layer) ulcerative tumors.

- Patients with target tumors that could potentially invade a major vascular
structure(s) (e.g., innominate artery, carotid artery), based on unequivocal imaging
findings, as determined by a radiologist.

- Patients with Grade ≥ 1 pre-existing neurologic abnormalities (CTCAE version 3.0).

- Patients who have been hospitalized for emergent conditions requiring inpatient
evaluation, treatment or procedure during the 30 days prior to entry on study. In
addition, emergent conditions requiring inpatient evaluation, treatment or procedure
must have resolved or be medically stable and not severe for 30 days prior to entry
on study.

- Patients with clinically evident Human Immunodeficiency Virus (HIV), Hepatitis B
Virus (HBV), Hepatitis C virus (HCV), or Epstein-Barr virus (EBV) infection.
Patients will be tested for HIV during pre-treatment screening.

- Patients receiving steroids or immunosuppressive agents, e.g., for rheumatoid
arthritis

- Concurrent use of any other investigational agents.

- Presence or history of central nervous system metastasis.

- Pregnant or breastfeeding women;women desiring to become pregnant within the
timeframe of the study are also excluded.

- Uncontrolled intercurrent illness including, but not limited to, ongoing or active
infection, symptomatic congestive heart failure, unstable angina pectoris, cardiac
arrhythmia, or psychiatric illness/social situations that would limit compliance with
study requirements.