The Impact of Omega Three Fatty Acids on Vascular Function in HIV

While omega-three fatty acids have been shown to be beneficial for TG and HDL-C levels in HIV
uninfected individuals and in some small, short duration studies in HIV-infected individuals,
there are no data that extend these observations to determine whether intake of omega-three
fats over a more prolonged time period will also have a beneficial impact on functional
outcomes such as vascular endothelial function and anatomic surrogate markers of CVD in
HIV-infected patients.

We propose a randomized, double blind trial of purified omega-three fatty acids in
HIV-infected individuals with elevated levels of triglycerides. While the impact of
omega-three fatty acids on lipid profiles should be evident early (within 12 weeks); we
propose to conduct this trial for a full 24 months to test our overall hypothesis that this
intervention will not only improve triglyceride and HDL-C levels, improve HDL-subpopulations,
plasma and membrane phospholipids and decrease inflammation, but will also improve brachial
artery reactivity testing (BART) as a measure of vascular endothelial function at 24 weeks
and 24 months and arterial stiffness measured by a pulse wave velocity test as a surrogate
marker of CVD risk at 24 months when compared to controls.

The specific aims of this proposal include:

1. To conduct a randomized, placebo controlled trial of omega-three fatty acids over 24
months in HIV-infected individuals with elevated levels of triglycerides (> 150 mg/dl).

2. To demonstrate the impact of omega-three fatty acid intake on TG levels and on HDL-C
levels, HDL subpopulations, composition of plasma and membrane phospholipids, and
chronic inflammation as measured by CRP, sPLA2 and by levels of arachidonic acid.

3. To demonstrate the impact of omega-three fatty acid intake on BART at 24 weeks and 24
months and on arterial stiffness at 24 months.

Inclusion Criteria:

- HIV-infected men and women at least 18 years of age,

- On stable HAART for the previous two months and without anticipated changes in their
HAART regimen throughout the duration of the study,

- Fasting triglycerides > 150 mg/dl and < 2,500 mg/dl

- Participants may be on lipid lowering therapy; if on lipid lowering therapy, therapy
must be stable for 8 weeks and cannot be changed during the course of the study.

- Participants may be on beta blockers (e.g., Atenolol, Metoprolol, Propranolol), and
Estrogens (e.g., Estinyl; Estrace; Estraderm), however therapy with these agents must
be stable for 8 weeks before starting the study and cannot be altered while on study
unless deemed medically necessary by the participant's medical provider and approved
by Dr. Wanke.

- Female participants of reproductive age must not be pregnant (negative test) or
lactating at screening and throughout the trial and agree to use contraception for the
course of the trial and 2 months after the trial unless they are surgically sterilized
(tubal ligation or hysterectomy), or post-menopausal with no menses for > 1 year.

- Ability to provide consent.

Exclusion Criteria:

- plasma HIV-1 RNA > 10,000 copies/ml

- change in HAART regimen over two months prior to study entry

- change in lipid lowering therapy within 2 months (8 weeks)

- Pregnancy in female participants

- Evidence of liver or renal disease with values of liver enzymes > 5 X upper limit of
normal or creatinine > 1.5 X upper limit of normal

- presence of active opportunistic infection or malignancy

- presence of other inflammatory or end organ disease (, rheumatoid arthritis, active
treatment for hepatitis c, or other diseases that may alter inflammatory markers)

- routine ingestion of fish oil (individuals who have used fish oil would be
reconsidered for study participation if they discontinue use of fish oil for 8 weeks
and TG levels remain elevated).

- Allergic to fish or Lovaza

- BMI >35