Pharmacokinetic Study in Patients With Chronic Kidney Disease and Healthy Volunteers

It has been demonstrated that proteins known as drug transporters in different human organs
and tissues are important for a drug to be absorbed, distributed, metabolized, and
eliminated (ADME)18. The chemical properties of drugs can affect whether it needs a
transporter protein to enter the cell or not. It is not well known how these proteins are
affected in chronic disease and how different drugs may be absorbed, metabolized, or
eliminated differently in certain diseases. Preliminary studies suggest that some drugs
(those requiring drug transporter proteins) may show altered elimination in the presence of
uremic toxins. Uremic toxins are substances accumulated in the blood of patients with
chronic kidney disease and many are not removed through hemodialysis (HD). We hypothesize
that the different classes of drugs (BDDCS class1, 2, and 3) will have different degrees of
changes in AUC, meaning that for a class 1 drug we would see less of a change in AUC than in
a class 3 drug because a class 3 drug requires transporters. Previous studies can't make
that comparison because they used different patients for each drug, so even if there were a
change in a class 1 drug, it can't be compared to a class 3 drug. In order to get an
accurate comparison, we will test the three drugs on the same patient and see how he AUC
changes from drug to drug within the same patient comparing it to the healthy volunteer
(taking the same three drugs).

Inclusion Criteria:

- Male or Female 18-70 years of age.

- Healthy volunteers or chronic kidney disease (GFR<40)

- Be able to provide written informed consent and comply with requirements of the
study.

- Avoid eating grapefruit and drinking grapefruit juice from 7 days prior to the first
study day until completion of the study.

- Abstinence from alcoholic beverages, caffeinated beverages and orange juice from 6pm
the night before a study day until completion of the study day.

- Fast from food and beverages at least 8 hours prior to medication dosing.

- Be able to read, speak, and understand English.

Exclusion Criteria:

- Subjects with contraindications to taking the study drugs

- Subjects with known allergies to propranolol, losartan, or eprosartan.

- Subjects who smoke tobacco.

- Subjects with ongoing alcohol or illegal drug use.

- Subjects who are pregnant, lactating, or attempting to conceive.

- Subjects unable to maintain adequate birth control during the study.

- Subjects unable to follow protocol instructions or protocol criteria.

- Subjects with hematocrit < 30mg/dL.

- Subjects who are insulin requiring diabetics.

- Subjects with low, or low normal blood pressure (systolic blood pressure [BP]
<100mmHg)

- Subjects with uncontrolled high blood pressure.