Bevacizumab, Chemotherapy and Valproic Acid in Advanced Sarcomas

Soft tissue sarcomas (STS) are a heterogeneous group of benign and malignant tumors of
various supportive tissues arising from the mesoderm. There are 56 known subtypes classified
by the tissue of origin. Soft tissue sarcomas account for 1% of all human malignancies. These
tumors share a common mesenchymal origin with the vasculature. Many of the signaling pathways
involved in angiogenesis also drive sarcoma tumor cell growth. Autocrine and paracrine
vascular endothelial growth factor (VEGF) - and platelet-derived growth factor
(PDGF)-mediated growth plays a role in the pathogenesis of several sarcoma subtypes1. Despite
promising preclinical data supporting a role for angiogenesis inhibition in sarcoma,
relatively few clinical trials have evaluated antiangiogenic therapy in sarcoma.

Most of the studies for the use of anti-angiogenic drugs have been taken from phase I trials
of refractory solid tumors showing a lack of response in sarcomas. This may be due to an
inadequate dose, wrong sequence of the treatment or lack of studies with combination
treatments. Given the heterogeneity of sarcomas there may be a benefit in certain subgroups
that can easily be missed. A recent clinical trial has shown improved cytotoxic cell kill and
response rates by epigenetically modifying the tumor environment prior to chemotherapy. It is
believed that the efficacy of angiogenesis inhibitors has been shown to be greatly improved
when they are combined with other anticancer drugs.

Tumor metastasis is highly dependent on angiogenesis progressing through a metastatic cascade
that includes primary tumor growth, modification of tumor cell growth and behavior and
formation of new blood vessels. This angiogenic switch is believed to be the result of
changes in the balance of angiogenesis stimulators, inhibitors and modulators present at the
site of the tumor growth. These changes are due to both genetic alterations involving RAS,
RAF, MYC, SRC, EGFR and HER-2 and tumor suppressor genes and epigenetic circumstances such as
hypoxia, inflammation or hormonal stimulation. It is clear that vascular endothelial growth
factor (VEGF) has emerged as the key stimulatory molecule for promoting angiogenesis in a
variety of human malignancies. Other pro-angiogenic factors can be induced or amplified in
the presence of hypoxia hypoglycemia, inflammatory cytokines and altered cell-cell contact.
Thus it would be of interest to combine epigenetic modifiers like Valproic acid, a histone
deacetylase inhibitor, with Bevacizumab, an anti-angiogenic agent against VEGF and standard
chemotherapy (Gemcitabine /docetaxel) to better modulate the cytotoxic effects against
sarcomas.

Inclusion Criteria:

1. Male or female patients aged ≥ 18 years old.

2. ECOG Performance Status of ≤ 2.

3. Ability to provide written informed consent obtained prior to participation in the
study and any related procedures being performed.

4. Patients must meet the following laboratory criteria:

Hematology: Neutrophil count of > 1500/mm3; Platelet count of >100,000/mm3L;
Hemoglobin ≥ 9 g/dL Biochemistry:AST/SGOT and ALT/SGPT ≤ 2.5 x upper limit of normal
(ULN) or ≤ 5.0 x ULN if the transaminase elevation is due to disease involvement;
Serum bilirubin ≤ 1.5 x ULN; Serum creatinine ≤ 1.5 x ULN or 24-hour creatinine
clearance ≥ 50 ml/min; Total serum calcium (corrected for serum albumin) or ionized
calcium ≥ LLN; Serum potassium ≥ LLN; Serum sodium ≥ LLN; Serum albumin ≥ LLN or
3g/dl; Patients with any elevated alkaline phosphatase due to bone metastasis can be
enrolled

5. Screening EKG with a QTc less than 450 msec confirmed by central laboratory prior to
enrollment to the study.

6. Baseline MUGA or ECHO done only in subjects with prior doxorubicin exposure. The test
must demonstrate LVEF ≥ the lower limit of the institutional normal.

7. Women of childbearing potential (WOCBP) must have a negative serum pregnancy test
within 7 days of the first administration of study treatment and must be willing to
use two methods of contraception one of them being a barrier method during the study
and for 3 months after last study drug administration

8. Any patient with the diagnosis of locally advanced, unresectable or metastatic sarcoma
from any site. These include untreated patients or those treated with chemotherapy 1st
line, 2nd line and 3rd line. Patients must have measurable disease defined as at least
1 lesion ≥ 1cm in the greatest dimension.

9. Previous exposure to Gemcitabine and Taxotere will only be allowed if there is no
residual toxicity from previous treatments. Toxicity must be graded as 0 or 1 prior to
study.

10. Patients must have had disease progression on or following their most recent treatment
regimen or on presentation for the first time with locally advanced unresectable or
metastatic disease.

1.All subtypes of sarcoma are eligible for the trial.

Exclusion Criteria:

1. Prior use of Bevacizumab for the treatment of cancer.

2. Prior HDAC, DAC, HSP90 inhibitors or valproic acid for the treatment of cancer.

3. Patients who will need valproic acid for any medical condition .

4. Inadequately controlled hypertension (defined as systolic blood pressure >150 mmHg
and/or diastolic blood pressure > 100 mmHg).

5. Prior history of hypertensive crisis or hypertensive encephalopathy.

6. New York Heart Association (NYHA) Grade II or greater congestive heart failure.

7. History of myocardial infarction or unstable angina within 6 months prior to Day 1.

8. History of stroke or transient ischemic attack within 6 months prior to Day 1.

9. Known CNS disease, except for treated brain metastasis: Treated brain metastases are
defined as having no evidence of progression or hemorrhage after treatment and no
ongoing requirement for dexamethasone, as ascertained by clinical examination and
brain imaging (MRI or CT) during the screening period. Anticonvulsants (stable dose)
are allowed. Treatment for brain metastases may include whole brain radiotherapy
(WBRT), radiosurgery (RS; Gamma Knife, LINAC, or equivalent) or a combination as
deemed appropriate by the treating physician. Patients with CNS metastases treated by
neurosurgical resection or brain biopsy performed within 3 months prior to Day 1 will
be excluded.

10. Significant vascular disease (e.g., aortic aneurysm, requiring surgical repair or
recent peripheral arterial thrombosis) within 6 months prior to Day 1

11. History of hemoptysis (>/= 1/2 teaspoon of bright red blood per episode) wit hin 1
month prior to Day 1

12. Evidence of bleeding diathesis or significant coagulopathy (in the absence of
therapeutic anticoagulation)

13. Core biopsy or other minor surgical procedure, excluding placement of a vascular
access device, within 7 days prior to Day 1

14. History of abdominal fistula or gastrointestinal perforation within 6 months prior to
Day 1

15. Serious, non-healing wound, active ulcer, or untreated bone fracture

16. Proteinuria as demonstrated by a UPC ratio >/= 1.0 at screening

17. Pregnancy (positive pregnancy test) or lactation. Use of effective means of
contraception (men and women) in subjects of child-bearing potential

18. Concomitant use of drugs with a risk of causing torsades de pointes

19. Concomitant use of CYP3A4 inhibitors

20. Other concurrent severe and/or uncontrolled medical conditions

21. Patients who have received chemotherapy or any investigational drug < 2 weeks prior to
starting study drug or who have not recovered from side effects of such therapy.

22. Major surgical procedure, open biopsy, or significant traumatic injury within 28 days
prior to Day 1 or anticipation of need for major surgical procedure during the course
of the study.

23. Concomitant use of any anti-cancer therapy or radiation therapy.

24. Male patients whose sexual partners are WOCBP not using a double method of
contraception during the study and 3 months after the end of treatment. One of these
methods must be a condom.

25. Patients with a history of another primary malignancy within 2 years other than
curatively treated CIS of the cervix, or basal or squamous cell carcinoma of the skin

26. Patients with known positivity for human immunodeficiency virus (HIV); baseline
testing for HIV is not required

27. Patients with any significant history of non-compliance to medical regimens or with
inability to grant a reliable informed consent

28. Bone sarcoma is excluded

29. Patients who are on drugs that prolong QT-interval on EKG (many antiarrhythmics,
tricyclics, phenothiazines, and others)