Fat Perception in Humans (09-0873)
| Status: | Completed |
|---|---|
| Conditions: | Obesity Weight Loss |
| Therapuetic Areas: | Endocrinology |
| Healthy: | No |
| Age Range: | 21 - 50 |
| Updated: | 7/19/2018 |
| Start Date: | May 2010 |
| End Date: | June 2014 |
Inhibition of Lipolysis in Oral Cavity and Fat Perception in Humans
There are many substances naturally present in the mouth that may help us taste fat in food.
Two of these substances (lipases and CD36) will be examined in this study.
The presence of fat in food increases food tastiness, therefore people often over-eat
high-fat foods and gain weight. The purpose of this study is to determine if blocking lipases
and some genetic variations in the CD36 gene will make fatty food less tasty so that people
eat less. Our hypothesis is that Orlistat and a particular gene will increase one's ability
to detect fat.
Two of these substances (lipases and CD36) will be examined in this study.
The presence of fat in food increases food tastiness, therefore people often over-eat
high-fat foods and gain weight. The purpose of this study is to determine if blocking lipases
and some genetic variations in the CD36 gene will make fatty food less tasty so that people
eat less. Our hypothesis is that Orlistat and a particular gene will increase one's ability
to detect fat.
1. Determine the effect of lipolysis inhibition in oral cavity on orosensory detection of
fat in humans.
Hypothesis: Orally applied Orlistat will increase detection thresholds of TAGs but not
FFA in humans.
To test this hypothesis we will measure triolein (a TAG) and oleic acid (a FFA) taste
detection thresholds with a three-alternative forced-choice (3-AFC) ascending
concentration method under two conditions (Orlistat day vs. Control day). To control for
olfactory input, subjects will be assessed wearing noseclips.
2. Determine the effect of CD36 variants on fat taste perception in humans. Hypothesis:
SNPs that associate with reduced CD36 expression will be associated with higher FFA and
TAG detection thresholds
To test this hypothesis we will measure triolein and oleic acid taste detection thresholds in
subjects who carry of the common CD36 e-SNP rs1761667 (i.e. a SNP that significantly reduces
CD36 level and has a minor allele frequency of 38-48%). We expect subjects who are at least
heterozygous for the expression reducing allele to have higher triolein and oleic acid taste
detection thresholds compared to non-carrier subjects.
fat in humans.
Hypothesis: Orally applied Orlistat will increase detection thresholds of TAGs but not
FFA in humans.
To test this hypothesis we will measure triolein (a TAG) and oleic acid (a FFA) taste
detection thresholds with a three-alternative forced-choice (3-AFC) ascending
concentration method under two conditions (Orlistat day vs. Control day). To control for
olfactory input, subjects will be assessed wearing noseclips.
2. Determine the effect of CD36 variants on fat taste perception in humans. Hypothesis:
SNPs that associate with reduced CD36 expression will be associated with higher FFA and
TAG detection thresholds
To test this hypothesis we will measure triolein and oleic acid taste detection thresholds in
subjects who carry of the common CD36 e-SNP rs1761667 (i.e. a SNP that significantly reduces
CD36 level and has a minor allele frequency of 38-48%). We expect subjects who are at least
heterozygous for the expression reducing allele to have higher triolein and oleic acid taste
detection thresholds compared to non-carrier subjects.
Inclusion Criteria:
- Body Mass Index greater than 30 kg/m²
Exclusion Criteria:
- smokers or who quit smoking less than six months ago
- pregnancy
- breastfeeding
- diabetes
- taking medications that might affect taste perception
- previous malabsorptive or restrictive intestinal surgery
We found this trial at
1
site
660 S Euclid Ave
Saint Louis, Missouri 63110
Saint Louis, Missouri 63110
(314) 362-5000
Washington University School of Medicine Washington University Physicians is the clinical practice of the School...
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