Gastrointestinal Dysfunction in Children Affected With Mitochondrial Disorders

Specific AIM: To study gastric emptying times in children with mitochondrial disorders.

INTRODUCTION: Mitochondrial disorders are a recently described group of metabolic disorders
with complex presentations; children present with a myriad of symptoms and involvement of a
wide array of organ systems. Mitochondrial disorders result from dysfunction of proteins or
ribosomes utilized by mitochondria. Mitochondrial dysfunction causes a lack of ATP
production and without enough ATP cells are unable to perform their biological functions.

The first mitochondrial disease was described in 1962. Over the last 40 years, mitochondrial
disease has become increasingly recognized as an important group of genetic disorders. The
prevalence has been reported to be as high as one in 3000 children, approaching that of
childhood cancer. Mitochondrial disorders have classically been described as affecting
tissues with the highest demand for ATP, i.e., brain, muscles, nerves, heart, and liver.
Increasing diagnosis of patients with mitochondrial disease has led to expansion of the
known spectrum of systemic involvement. Many children with mitochondrial disorders have
gastrointestinal manifestations, predominately constipation and poor gastrointestinal
motility. Poor motility effects feeding and many of these patients are intolerant to oral
feeding and require mechanical feeding to survive.

Currently there are no proven treatment strategies for children with mitochondrial
disorders. Most of the current management strategies are nonspecific and target symptom
control with only limited utility and success.

Study Methods: We plan to conduct a large, prospective cohort study of 25 children with
mitochondrial disorders. Subjects will include children with different subclasses of
various mitochondrial disease subgroups who meet the modified Walker criteria for diagnosis
of a probable or definite mitochondrial disorder.

Gastric emptying times will be measured to assess gastrointestinal motility, function, and
dynamics.

Research Design and Methods: Participants for this study will be recruited prospectively
through the University of Texas (UT) Mitochondrial Clinic. All mitochondrial patients with
probable or definite mitochondrial disorders from 3 to 18 years of age seen at the
University of Texas Mitochondrial Clinic will be invited to participate. The decision to
participate or not to participate in this research study will in no way affect medical care
offered by clinicians involved in the UT Mitochondrial Clinic.

Consent for study participation will be obtained by the Principal Investigator (PI) or
co-investigator (CI) or designated clinical nurse in person and a copy of the signed consent
form will be retained on file by the PI, placed in the patient's research medical record
(separate from the clinic record). For minor participation (12 to 17 years) parental
permission and consent of the child will be obtained. For minor participation (7 to 11
years) parental permission and assent of the child will be obtained. For minor participation
(birth to 6 years), parental permission will be obtained

This study population consists of a cohort of patients with different subsets of
mitochondrial disorders, including children with isolated deficiencies of the electron
transport chain and children with syndromic mitochondrial disorders. Final analysis will
will evaluate results both individually and in subclass groups, ie, Complex I deficiency,
MELAS (mitochondrial encephalopathy lactic acidosis and stroke-like syndrome), etc.

Research design: Patients will undergo testing for gastric emptying scans at our radiology
department. The studies would be performed using Technetium-99 as tracer by our UT
radiologist David Wan, MD. Patient would report on the morning of the study NPO and would
drink the tracer. The transit of the contrast material through the stomach would be
monitored and evaluated with regularly scheduled X Rays of the stomach and the final results
would be interpreted as normal, delayed or significantly delayed.

Risks and Benefits : The risks of this study are largely confined to maintaining patient
confidentiality. There will be no personal benefit to the participant/family. No payment
will be offered in exchange for study participation. Successful development of a patient
database will provide valuable information on the natural history of these complicated
disorders.

Information derived from the database will be published in a peer reviewed medical journal;
however no individual would be identified and all personal information would be kept
confidential. A potential benefit to the scientific community will be greater understanding
of the clinical course and expected laboratory values observed in persons with mitochondrial
disease. There are no foreseeable risks to the research staff.

Confidentiality: All participants' names and other information will be kept strictly
confidential. All participants will be assigned a subject ID number and an individual
identification number. The code linking name and subject ID number will be maintained in a
locked file in the office of the PI and in a spread sheet file on the office computer of the
PI. All subsequent reference to patient data will be in anonymous form by patient
identification number.

Determine if gastric emptying times in children with mitochondrial disorders are abnormal.

There are no known risks of performing gastric emptying scan studies in children.

The risk of allergy to eggs/food is similar to that observed in other children of similar
age groups and is minimal.

RISKS:

Risk associated with the allergic reaction to the dye is also similar to the one observed in
other children of similar age groups and is minimal.

Risk associated with breach of confidentiality- minimal. The names and other identification
information pertinent to the patient's identity and test results would be strictly kept
confidential and patients would be never identified by their names or other pieces of
information in public or to anyone else not related to the study.

Patient's test results would also be not released to the insuring companies directly or
indirectly.

Risk associated with the procedure- The procedure itself is very safe and has been performed
in children of all age groups including the ones suffering from chronic disorders like the
mitochondrial disorders all the time. Our radiologists are familiar with the nature of
medical disorder that these children presents with and are very comfortable performing this
procedure in a safe and timely manner.