EBV CTLs Expressing CD30 Chimeric Receptors For CD 30+ Lymphoma
| Status: | Active, not recruiting |
|---|---|
| Conditions: | Lymphoma |
| Therapuetic Areas: | Oncology |
| Healthy: | No |
| Age Range: | Any |
| Updated: | 2/20/2019 |
| Start Date: | May 10, 2011 |
| End Date: | October 2033 |
Phase I Study of the Administration of EBV CTLs Expressing CD30 Chimeric Receptors for Relapsed CD30+ Hodgkin's Lymphoma and CD30+ Non-Hodgkin's Lymphoma (CAR CD 30)
The body has different ways of fighting infection and disease. No single way seems perfect
for fighting cancer. This research study combines two different ways of fighting disease:
antibodies and T cells. Antibodies are proteins the protect the body from diseases caused by
germs or toxic substances. They work by binding those germs or substances, which stops them
from growing and causing bad effects. T cells, also called T lymphocytes, are special
infection-fighting blood cells that can kill other cells, including tumor cells or cells that
are infected with germs. Both antibodies and T cells have been used to treat patients with
cancers: they both have been shown promise, but have not been strong enough to cure most
patients. This study combines the two methods.
We have found from previous research that we can put a new gene into T cells that will make
them recognize cancer cells and kill them. We now want to see if we can attach a new gene to
T cells that will help them do a better job at recognizing and killing lymphoma cells.
The new gene we will put in T cells makes an antibody called anti-CD30. The antibody alone
has not been strong enough to cure most patients. For this study, the anti-CD30 antibody has
been changed so that instead of floating free in the blood it is now joined to the T cells.
When an antibody is joined to a T cell in this way it is called a chimeric receptor. These
chimeric receptor-T cells seem to kill some of the tumor, but they don't last very long and
so their chances of fighting the cancer are unknown.
We have found that T cells that are also trained to recognize the EBV virus (that causes
infectious mononucleosis) can stay in the blood stream for many years. These are called EBV
specific Cytotoxic T Lymphocytes.
By joining the anti-CD30 antibody to the EBV CTLs, we believe that we will also be able to
make a cell that can last a long time in the body and recognize and kill lymphoma cells. We
call the final cells CD30 chimeric receptor EBV CTLs. T
We hope that these new cells may be able to work longer and target and kill lymphoma cells.
However, we do not know that yet.
for fighting cancer. This research study combines two different ways of fighting disease:
antibodies and T cells. Antibodies are proteins the protect the body from diseases caused by
germs or toxic substances. They work by binding those germs or substances, which stops them
from growing and causing bad effects. T cells, also called T lymphocytes, are special
infection-fighting blood cells that can kill other cells, including tumor cells or cells that
are infected with germs. Both antibodies and T cells have been used to treat patients with
cancers: they both have been shown promise, but have not been strong enough to cure most
patients. This study combines the two methods.
We have found from previous research that we can put a new gene into T cells that will make
them recognize cancer cells and kill them. We now want to see if we can attach a new gene to
T cells that will help them do a better job at recognizing and killing lymphoma cells.
The new gene we will put in T cells makes an antibody called anti-CD30. The antibody alone
has not been strong enough to cure most patients. For this study, the anti-CD30 antibody has
been changed so that instead of floating free in the blood it is now joined to the T cells.
When an antibody is joined to a T cell in this way it is called a chimeric receptor. These
chimeric receptor-T cells seem to kill some of the tumor, but they don't last very long and
so their chances of fighting the cancer are unknown.
We have found that T cells that are also trained to recognize the EBV virus (that causes
infectious mononucleosis) can stay in the blood stream for many years. These are called EBV
specific Cytotoxic T Lymphocytes.
By joining the anti-CD30 antibody to the EBV CTLs, we believe that we will also be able to
make a cell that can last a long time in the body and recognize and kill lymphoma cells. We
call the final cells CD30 chimeric receptor EBV CTLs. T
We hope that these new cells may be able to work longer and target and kill lymphoma cells.
However, we do not know that yet.
The EBV CTLs will be made for specific patients. First blood will be collected from the
patient and then the the CD30 chimeric-EBV CTLs will be created in the lab. The cells will
then be grown and frozen for the patient.
To get the CD30 antibody to attach to the surface of the T cell, the lab will insert the
antibody gene into the T cell. This is done with a virus called a retrovirus that has been
made for this study and will carry the antibody gene into the T cell. Because the patient
will have received cells with a new gene in them they will be followed for a total of 15
years to see if there are any long term side effects of gene transfer.
When the patient is enrolled on this study, they will be assigned to one of the following
dose levels of CD30 chimeric receptor-EBV CTLs.
- 2×10^7 cells/m2
- 5x10^7 cells/m2
- 1×10^8 cells/m2
The dose level of cells that they will receive will not be based on a medical determination
of what is best for them, instead the dose is based on the order in which the patient enrolls
on the study relative to other participants. Subjects enrolled earlier in the study will
receive a lower dose of cells than those enrolled later in the study. The risks of harm and
discomfort from the study treatment may bear some relationship to the dose level. The
potential for direct benefit, if any, may also vary with the dose level. To enroll on this
study they will need to have recovered from toxic effects of previous chemotherapy for at
least one week and not be receiving any other investigational agents. Patients cannot have
received any tumor vaccines within the previous six weeks.
patient and then the the CD30 chimeric-EBV CTLs will be created in the lab. The cells will
then be grown and frozen for the patient.
To get the CD30 antibody to attach to the surface of the T cell, the lab will insert the
antibody gene into the T cell. This is done with a virus called a retrovirus that has been
made for this study and will carry the antibody gene into the T cell. Because the patient
will have received cells with a new gene in them they will be followed for a total of 15
years to see if there are any long term side effects of gene transfer.
When the patient is enrolled on this study, they will be assigned to one of the following
dose levels of CD30 chimeric receptor-EBV CTLs.
- 2×10^7 cells/m2
- 5x10^7 cells/m2
- 1×10^8 cells/m2
The dose level of cells that they will receive will not be based on a medical determination
of what is best for them, instead the dose is based on the order in which the patient enrolls
on the study relative to other participants. Subjects enrolled earlier in the study will
receive a lower dose of cells than those enrolled later in the study. The risks of harm and
discomfort from the study treatment may bear some relationship to the dose level. The
potential for direct benefit, if any, may also vary with the dose level. To enroll on this
study they will need to have recovered from toxic effects of previous chemotherapy for at
least one week and not be receiving any other investigational agents. Patients cannot have
received any tumor vaccines within the previous six weeks.
INCLUSION CRITERIA:
PROCUREMENT Inclusion Criteria:
- Referred patients will initially be consented for procurement of blood for generation
of the transduced CTL Line. Eligibility criteria at this stage include:
- Diagnosis of recurrent HL or NHL, or newly diagnosed patients unable to receive or
complete standard therapy OR diagnosis of relapsed/refractory HL or NHL with a
treatment plan that will include high dose therapy and stem cell transplantation
- CD30 positive tumor (can be pending at this time)
- EBV seropositivity (can be pending at this time)
- Hgb > 8.0
- Informed consent explained to, understood by and signed by patient/guardian.
Patient/guardian given copy of informed consent.
TREAMENT Inclusion Criteria: Patients must meet the following eligibility criteria to be
included for treatment:
- Diagnosis - CD30+ HL or CD30+ NHL
- During the dose escalation phase: only adult patients (age 18 and older) with active
disease failing standard therapy
- After dose escalation: any patient (children or adults) with relapsed CD30+ HL or
CD30+ NHL or newly diagnosed patients unable to receive or complete standard therapy
OR diagnosis of relapsed/refractory CD30+ HL or CD30+ NHL with a treatment plan that
will include high dose therapy and autologous stem cell transplantation.
- CD30 positive tumor
- EBV seropositivity.
- Recovered from acute toxic effects of all prior chemotherapy at least one week and 30
days from prior chemotherapy before entering this study.
- Bilirubin 1.5 times or less than upper limit of normal.
- AST 3 times or less than upper limit of normal.
- Serum creatinine 1.5 times or less than upper limit of normal.
- Pulse oximetry of > 90% on room air
- Karnofsky or Lansky score of > 60%.
- Available autologous transduced EBV-specific cytotoxic T lymphocytes with 15% or more
expression of CD30CAR determined by flow-cytometry.
- Adequate pulmonary function with FEV1, FVC and DLCO 50% or greater of expected
corrected for hemoglobin. Exceptions may be allowed for patients with pulmonary
involvement after discussing with PI.
- Sexually active patients must be willing to utilize one of the more effective birth
control methods during the study and for 6 months after the study is concluded. The
male partner should use a condom.
- Patients or legal guardians must sign an informed consent indicating that they are
aware this is a research study and have been told of its possible benefits and toxic
side effects. Patients or their guardians will be given a copy of the consent form.
EXCLUSION CRITERIA:
PROCUREMENT Exclusion Criteria:
- Active infection with HIV, HTLV, HBV, HCV (can be pending at this time).
- Received rituximab within 4 months of blood collection for LCL initiation (unless
circulating CD19+ B are =/>2%)
TREATMENT Exclusion Criteria:
- Currently receiving any investigational agents or received any tumor vaccines within
the previous six weeks.
- Received anti-CD30 antibody-based therapy within the previous 6 weeks.
- History of hypersensitivity reactions to murine protein-containing products.
- Pregnant or lactating.
- Tumor in a location where enlargement could cause airway obstruction.
- Current use of systemic corticosteroids.
We found this trial at
2
sites
Texas Children's Hospital Texas Children's Hospital, located in Houston, Texas, is a not-for-profit organization whose...
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Houston Methodist Hospital Houston Methodist is comprised of a leading academic medical center in the...
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