Bendamustine and Dexamethasone in Patients With Relapsed AL Amyloidosis

Systemic light-chain amyloidosis (AL) is a protein conformation disorder due to a clonal
plasma cell dyscrasia. There are no established and approved second-line therapies for
patients with systemic AL amyloidosis who fail initial melphalan-based treatment, be it
high-dose melphalan with stem cell transplant or oral melphalan and dexamethasone (MDex).
Therefore new treatments are needed for those who fail initial therapy and for those who
initially respond but subsequently relapse.

Therapy of AL is generally based on treatment regimens used in multiple myeloma (MM).
Bendamustine achieves partial response with relapsed/refractory MM. Based on this high
anti-MM activity, we anticipate that bendamustine will also be very active in clonal plasma
cell disorder associated with AL.

Inclusion Criteria:

- Male or female patients aged ≥ 18 years old

- Histopathology of amyloidosis or light chain deposition disease based on detection by
polarizing microscopy of green bi-refringent material in Congo red-stained tissue
specimens or characteristic electron microscopy appearance or immunohistochemical
stain with anti-light chain anti-sera

- Demonstrate measurable disease as defined by one or more of the following:

- Serum monoclonal protein ≥ 0.5 g/dL by serum electrophoresis

- Urine monoclonal protein > 200 mg/dL in a 24 hr urine electrophoresis

- Serum immunoglobulin free light chain ≥ 5 mg/dL and abnormal serum immunoglobulin
kappa lambda free light chain ratio. The difference between involved and
uninvolved free light chains should be ≥ 5 mg/dL (dFLC)

- Demonstrate clonal population of plasma cells in the bone marrow or
immunohistochemical stain with anti-light chain anti-sera of amyloid fibrils

- Eastern Cooperative Oncology Group (ECOG) performance status of 0, 1, or 2

- Patients had at least one prior regimen consisting of at least 1 cycle

- If not previously transplanted, patient should be either ineligible for autologous
stem cell transplantation (ASCT), or must have declined the option of ASCT. Patients
who have previously had ASCT and have subsequently progressed are eligible, provided
other entry criteria are met

- Ability to provide written informed consent obtained prior to participation in the
study and any related procedures being performed

Patients must meet the following laboratory criteria:

- Absolute neutrophil count (ANC) ≥ 1.5 x 10^9/L

- Hemoglobin ≥ 9 g/dl (May transfuse packed red blood cells (PRBC) to meet parameter)

- Platelets ≥ 100x 10^9/L (Must be independent of platelet transfusion)

- Calculated creatinine clearance (CrCl) greater than or equal to 30 mL/min
(Cockcroft-Gault Formula )

- Aspartate transaminase (AST) and alanine transaminase (ALT) ≤ 2.5 x upper limit of
normal (ULN)

- Serum bilirubin <1.5 x ULN

- Serum potassium within normal limits

- Total serum calcium (corrected for serum albumin) or ionized calcium ≤ ULN

Exclusion Criteria:

- Patients meeting the criteria for symptomatic MM:

- Lytic lesions on skeletal survey or plasmacytoma

Patients meeting International Myeloma Working Group definition of symptomatic myeloma with
symptoms only related to associated amyloidosis who would otherwise only meet the criteria
for smoldering MM are potentially eligible

- Myocardial infarction within 6 months prior to enrollment or has New York Heart
Association (NYHA) Class III or IV heart failure uncontrolled angina, severe
uncontrolled ventricular arrhythmias, or

- electrocardiographic evidence of acute ischemia or active conduction system
abnormalities (not including 1st degree atrioventricular (AV)-block, Wenckebach type
2nd degree heart block, or left bundle branch block. Prior to study entry, any
electrocardiogram (ECG) abnormality at Screening has to be documented by the
investigator or an authorized physician sub-investigator as not medically relevant).
Note: There is no lower limit of left ventricular ejection fraction below which
patients are excluded from participation.

- Patients with N-terminal (NT)-proBNP ≥ 1800nb/L or B-type natriuretic peptide (BNP) ≥
400 ng/L, abnormal cardiac troponin T (cTnT) or cardiac troponin l (cTnI)

- Patient has received other investigational drugs within 14 days prior to enrollment

- Any form of secondary / familial amyloidosis

- Serious concurrent illness, which in the opinion of the investigator or an authorized
physician sub-investigator would interfere with participation in this clinical study,

- Known HIV infection.

- Inability to provide informed consent or to comply with the schedule of office and
treatment visits

- Female subject is pregnant or breast-feeding. Confirmation that the subject is not
pregnant must be established by a negative serum beta-human chorionic gonadotropin
pregnancy test result obtained during screening. Pregnancy testing is not required for
post-menopausal or surgically sterilized women(woman not of child-bearing potential is
defined as any woman whose menstrual periods have stopped in the past 12 consecutive
months or have had a complete hysterectomy or both ovaries surgically removed).

- Diagnosed or treated for another malignancy within 3 years of enrollment, with the
exception of complete resection of basal cell carcinoma or squamous cell carcinoma of
the skin, an in situ malignancy, low-risk prostate cancer, or cancer after curative
treatment.