Evaluating the Safety of and Immune Response to an HIV Vaccine Followed by Booster, Administered by Two Devices, in HIV-Uninfected Adults

Despite advances in treatment, HIV/AIDS rates remain high in low- and middle-income
countries in resource-limited areas of the world. Preventive HIV vaccines would be an
effective way to decrease the spread of HIV/AIDS. This study will evaluate an experimental
HIV preventive vaccine followed by booster: the PENNVAX-G DNA vaccine and the Modified
Vaccinia Ankara-Chiang Mai Double Recombinant (MVA-CMDR) booster vaccine. The MVA virus is a
mild form of a vaccinia virus that is used in smallpox vaccines and does not cause smallpox
infection. It has been safely used in vaccines for many years. Study researchers will
examine if the combination of the two vaccines will provide an effective way for the body to
build a defense against HIV. The PENNVAX-G vaccine will be administered to participants
using one of two devices—the Biojector 2000 needleless device or the CELLECTRA intramuscular
(IM) electroporation (EP) device—to evaluate how the immune response to the vaccine changes
based on the device that is used. The purpose of this study is to evaluate the safety and
immunogenicity of the PENNVAX-G vaccine, administered by either Biojector 2000 injection or
CELLECTRA EP, followed by an MVA-CMDR vaccine boost, in healthy, HIV-uninfected adults.

This study will be conducted in two parts. Participants in the first part of the study will
attend a baseline study visit, and they will undergo blood and urine collection, a medical
history review, physical examination, and HIV testing counseling. All participants will
receive the PENNVAX-G vaccine at baseline and Day 28. They will be randomly assigned to
receive the vaccine by either the Bioinjector 2000 needleless device or the CELLECTRA IM EP
device. On Days 84 and 168, all participants will receive the MVA-CMDR vaccine by IM
injection delivered via needle and syringe. Participants will remain in the clinic for 1
hour after each vaccination for observation and vital sign monitoring, and they will record
their temperatures and any symptoms in a diary for 7 days after each vaccination. Study
staff will contact participants 1 to 2 days after each vaccination for follow-up monitoring.
In addition to the vaccination study visits, participants will attend study visits at Weeks
1, 2, 4, 5, 6, 13, 14, 25, 26, 37, 50, and 52 and will repeat the baseline study procedures.

At the conclusion of the first part of the study, participants' study data will be reviewed
and the safety of the vaccine regimen will be determined. If the regimen is found to be
safe, then the second part of the study will begin. Participants in the second part of the
study will be randomly assigned to receive the PENNVAX-G vaccine administered by either the
Bioinjector 2000 device or the CELLECTRA EP device at baseline and Day 28, followed by the
MVA-CDMR boost at Days 84 and 168, or they will receive placebo PENNVAX-G vaccine
administered by either the Bioinjector 2000 device or the CELLECTRA EP device at baseline
and Day 28, followed by a placebo MVA-CDMR boost at Days 84 and 168. All study visits and
procedures that occurred during the first part of the study will also take place in the
second part of the study.

Participants in the second part of the study will have the option of enrolling in a
substudy. The purpose of the substudy is to evaluate the antibody response of the
reproductive tract mucosa to the PENNVAX-G DNA, MVA-CDMR, and placebo vaccines administered
in the main part of study. The substudy will also evaluate the effectiveness of using the
Instead Softcup as a collection method to collect vaginal secretions for testing. Mucosal
specimens and urine samples will be collected from participants at the screening visit and
during the Week 2, 6, 14, 26, and 50 study visits.

Inclusion Criteria:

- Low risk of HIV infection (as defined by the Study Risk Assessment Tool captured
during the medical history)

- Amenable to HIV risk reduction counseling, committed to maintaining behavior
consistent with low risk of HIV exposure through the last required protocol clinic
visit, and committed to 18 months of follow-up contact

- Pass the Test of Understanding and demonstrate an understanding of STEP study results
(HVTN 502/Merck 023 trial)

- Assessed by the clinic staff as being at low risk of HIV infection on the basis of
sexual behaviors within the 12 months prior to enrollment as follows: sexually
abstinent, or had two or fewer mutually monogamous relationships with HIV-uninfected
partners and who have not used illicit drugs, or had two or fewer partners believed
to be HIV uninfected and who did not use illicit drugs and with whom he/she regularly
uses condoms for sexual intercourse

- Healthy men and women (determined by medical history, physical examination, and
clinical judgment)

- Available and willing to participate for 12 months for study visits and annual
follow-up for 18 months after study completion

- Must be willing to have photo or fingerprint taken for identification purposes

- Must be willing to be taken home at enrollment visit and allow home visits, if needed

- Able to read and willing to complete the informed consent process

- Has the following laboratory criteria within 45 days prior to study entry:

1. Hemoglobin: Women: 11 mg/dL; Men: 12.5 mg/dL

2. White cell count: 2,500 to 11,000 cells/mm^3

3. Platelets: 125,000 to 450,000 per mm^3

4. Urinalysis: protein and blood less than 1+, glucose negative

5. Normal liver function tests to include alanine aminotransferase (ALT)/aspartate
aminotransferase (AST), alkaline phosphatase, gamma-glutamyl transpeptidase
(GGT) (less than or equal to 1.25 times the institutional upper limits of
normal), creatine phosphokinase (CPK) (less than or equal to 600 IU/L), troponin
I (less than 0.4 ng/mL), and creatinine (less than or equal to 1.25 times the
institutional upper limits of normal)

6. Negative for HIV infection (enzyme linked immunosorbent assay [ELISA], Western
blot [WB], and HIV polymerase chain reaction [PCR])

- Female participants must have a negative pregnancy test at the screening visit and
have a negative pregnancy test immediately prior to each vaccine/placebo vaccination

- Provide verbal assurance that adequate birth control measures have been followed for
45 days prior to the first vaccine/placebo vaccination and will continue to be
followed for at least 3 months after the final vaccine/placebo vaccination. More
information on this criterion can be found in the protocol.

- Body mass index (BMI) less than 30

Exclusion Criteria:

- Confirmed HIV-1 or HIV-2 infection

- Engaged in excessive daily alcohol use, frequent binge drinking, or illicit drug use
within the 12 months prior to study entry

- History of new onset, sexually acquired infection, as determined by local, syndromic
diagnostics standards or, as available, serologic and microbiologic diagnosis within
the 12 months prior to study entry

- Has a known current high-risk partner or had such a partner within the 12 months
prior to study entry

- Hepatitis B, hepatitis C, or syphilis infection; active syphilis documented by exam
or serology unless positive serology is because of remote treated infection or
positive rapid plasma reagin

- Pregnant, planning on becoming pregnant during the study, or breastfeeding

- Any clinically significant acute or chronic medical condition that, in the opinion of
the investigator, would preclude study participation (e.g., history of seizure
disorders, bleeding/clotting disorder, autoimmune disease, malignancy, tuberculosis,
other systemic infections)

- Major surgery within the 4 weeks prior to study entry

- History of or known active heart disease including:

1. Previous myocardial infarction (heart attack)

2. Angina pectoris; congestive heart failure

3. Valvular heart disease, including mitral valve prolapse

4. Cardiomyopathy

5. Myo/pericarditis

6. Stroke or transient ischemic attack

7. Chest pain or shortness of breath with activity (such as walking up stairs)

8. Arrhythmia/episodic palpitations (not excluded: sinus arrhythmia)

9. Pacemaker

10. Other heart conditions under the care of a doctor

- People who have the following cardiac risk factors:

1. Participant report of history of elevated blood cholesterol defined as fasting
low-density lipoprotein (LDL) greater than 160 mg/dL

2. First degree relative (e.g., mother, father, brother, sister) who had coronary
artery disease before the age of 50 years

- Electrocardiogram (ECG) with clinically significant findings, or features that would
interfere with the assessment of myo/pericarditis. More information on this criterion
can be found in the protocol.

- History of diabetes mellitus type 1 or type 2, including cases controlled with diet
alone (not excluded: history of isolated gestational diabetes)

- Thyroidectomy or thyroid disease requiring medication during the 12 months prior to
study entry

- High blood pressure:

1. If a person has been diagnosed with high blood pressure during screening or
previously, exclude for high blood pressure that is not well controlled. More
information on this criterion can be found in the protocol.

2. If a person has NOT been diagnosed with high blood pressure during screening or
previously, exclude for systolic blood pressure greater than or equal to 150 mm
Hg at study entry or diastolic blood pressure greater than or equal to 100 mm Hg
at study entry

- Major psychiatric illness and/or substance abuse problems during the 12 months before
study entry that, in the opinion of the investigator, would preclude study
participation

- Receipt of live attenuated vaccine within 30 days or inactivated/killed vaccine
within 2 weeks of DNA vaccination

- Use of experimental therapeutic agents within 30 days of study entry

- Current or planned participation in another clinical study during the study period

- Receipt of blood products or immunoglobulin in the 3 months before study entry

- History of anaphylaxis or other serious adverse reactions to vaccines or egg products
or amide type anesthetics (e.g., bupivacaine, novocaine, lidocaine, mepivacaine,
neomycin, streptomycin)

- History of chronic urticaria (recurrent hives)

- Chronic or recurrent use of medications that modify host immune response (e.g.,
cancer chemotherapeutic agents, parenteral corticosteroids [topical not an
exclusion])

- Recipient of an HIV vaccine candidate at any time and receipt of other experimental
vaccine(s) within the 5 years before study entry. More information on this criterion
can be found in the protocol.

- A study site employee

- Military personnel (will be excluded from participation in this study at all sites
due to the potential for a false-positive HIV test result on mandatory HIV testing,
which could have adverse affects on deployment status)