Alterations in Protein Synthesis Rates of Burn Patients Measured Over Time Using PET Scans

The purpose of this study is to understand how muscles are built up and broken down
(metabolism) in both burn patients and healthy people.The studies will help us understand
how muscle metabolism is different for burn patients and healthy subjects. We hope to find
ways to help the body of a burn victim heal without losing too much muscle.

Protein is an important part of our daily diet. Normally, the body breaks down the protein
we eat into smaller pieces called amino acids. It uses the amino acids to make its own
proteins. It uses these proteins to heal wounds, fight infections, and provide energy.

After a burn injury, the body speeds up the pace of its life activities. It needs more
energy just to stay alive and recover from the burn. The body often gets the extra energy it
needs by breaking the protein in muscles down into amino acids. The amino acids are then
made into new proteins. If too much muscle is broken down, it is harder for the body to
function. This can make recovery slower or possibly lead to death.

For Healthy Volunteers, this study will require no more than 6 hours of time in total. This
time will be spread out over two visits, a Screening Visit and a Scan Visit.

For Burn Patients, this study will require no more than 14 hours total. This time will be
spread out over four visits-a screening visit and a maximum of 3 scan visits. A total of
three PET scans will be done over a 2 year period.

Inclusion Criteria:

FOR HEALTHY VOLUNTEERS

Healthy males and females 18-70 years of age.

Laboratory results within MGH-accepted normal range.

Within 20% of the ideal weight for their height.

Supine and standing blood pressure within the range of 110/60 to 150/90 mm Hg

Heart rate within the range of 46-90 beats/minute after 5 minutes of rest.

Subjects <40 years old with HCT >=24, age 40-60 with HCT >=27, age 60-70 with HCT >=30.

FOR BURN SUBJECTS:

Inclusion Criteria:

Burn Injury of >=5 % TBSA from any cause.

18 -70 years of age

Stable hemodynamic and cardiopulmonary states as judged by the attending surgeons in the
burn unit.

Patient who is capable of giving full informed consent.

Exclusion Criteria:

FOR HEALTHY SUBJECTS Clinical evidence of physical or mental disease.

Clinically significant abnormality of the laboratory tests.

Known drug or alcohol dependence.

History of drug allergy

Taking standard prescription drugs within two weeks or investigational drugs within four
weeks prior to the PET scan

Diabetes mellitus and other metabolic endocrine disorders Pregnant or lactating

FOR BURN SUBJECTS:

Pregnant or lactating females