Specimen Collections From Patients With HIV Infection, KSHV Infection, Viral-Related Pre-malignant Lesions and Cancer

BACKGROUND:

-A number of important scientific advances can be made through the study of blood, bone
marrow, tumor, or other tissue samples from patients with HIV infection, infection with
Kaposi s sarcoma associated herpesvirus (KSHV), infection with other oncogenic viruses, or
cancer.

This protocol provides a mechanism to effect a variety of such studies.

OBJECTIVES:

-Acquisition of serum, circulating cells, bone marrow, and tumor or normal tissue samples
from patients with HIV infection, KSHV infection, or with cancer.

ELIGIBILITY:

-Eligibility criteria include age 18 years or older and at least one of the following:
Exposure risk to HIV, KSHV, or HPV; HIV seropositive; KSHV seropositive; EBV seropositive;
HTLV-1 seropositive; malignancy, Castleman s disease, or skin lesions with appearance of
Kaposi s sarcoma; or cervical or anal intraepithelial lesion.

DESIGN:

- Blood samples may be collected at the initial visit, and at follow-up visits.

- Tumor samples may be obtained by fine needle aspirate, by removal of pleural or
peritoneal fluid, by skin punch biopsy, or by excisional biopsy, providing the tumor is
accessible with minimal risk to the patients.

- Specific risks will be described in a separate consent to be obtained at the time of the
biopsy.

- Samples will be studied in the HIV and AIDS Malignancy Branch, CCR, NCI; laboratories in
NCI-Frederick; or those of collaborating investigators.

- INCLUSION CRITERIA:

Age 18 years or older.

ECOG performance status less than or equal to 3.

At least one of the following: Exposure risk to HIV, KSHV, or HPV; HIV seropositive; KSHV
seropositive; EBV seropositive; HTLV-1 seropositive; Malignancy, Castleman's disease, or
skin lesions with appearance of Kaposi's sarcoma; Cervical or anal intraepithelial lesion;
Clinical condition reported or hypothesized to be associated with xenotropic murine
leukemia virus-relted virus (XMRV)

Patients not requiring biopsy for a medical indication can be included on the study solely
for the purpose of obtaining research samples, including blood, pleural fluid, peritoneal
fluid, or biopsy samples providing the biopsy or aspiration procedure is of low risk: such
procedure include fine needle aspirates, bone marrow aspirate and biopsy, or excisional
biopsy. Such biopsies can be completely separate from any other protocol or evaluation
process.

EXCLUSION CRITERIA:

Inability to provide informed consent.