Phase 1-2 Amrubicin in Combo With Lenalidomide + Weekly Dexamethasone in Relapsed/Refractory Multiple Myeloma

PRIMARY OBJECTIVES

- Establish the maximum tolerated dose (MTD) and toxicity profile for the combination of
amrubicin with lenalidomide and dexamethasone in previously treated adult patients with
multiple myeloma during Phase I

- Determine the combined rate of complete response (CR) and very good partial response
(VGPR) for this combination in this population as defined by the International Myeloma
Working Group Uniform Response Criteria (IMWGURC)

SECONDARY OBJECTIVES

- Determine the overall response rate (CR, VGPR and PR)

- Assess additional evidence of ant-tumor activity as measured by duration of response
(DOR), progression-free survival (PFS), time to tumor progression (TTP), and overall
survival (OS)

Inclusion Criteria:

- Relapsed or refractory multiple myeloma that has progressed following at least 1 prior
therapy.

- Measurable disease defined as one of the following:

- Serum M-protein ≥ 1 g/dL

- Urine M-protein ≥ 200 mg/24 hours

- Received at least 1 prior line of systemic treatment that may have included
lenalidomide and/or an anthracycline.

- No cytotoxic chemotherapy within 4 weeks prior to first dose of amrubicin. This
interval may be reduced to 14 days for thalidomide, lenalidomide, bortezomib or
corticosteroids, provided other entry criteria are met.

- Age ≥ 18 at the time of consent.

- Life expectancy of more than ≥ 3 months.

- No known central nervous system involvement by myeloma.

- Eastern Cooperative Oncology Group (ECOG) performance status 0 to 1 at study
registration during phase 1. Once safety is confirmed, ECOG performance status 0 to 2
at study registration during phase 2.

- No poorly-controlled intercurrent illness.

- Platelets > 100 x 10^9/L

- Hemoglobin > 8.0g/dL

- Absolute neutrophil count (ANC) >1.5 x 10^9/L

- Aspartate transaminase (AST) and alanine transaminase (ALT) ≤ 3 x upper limit of
normal (ULN)

- Total bilirubin ≤ 1.5 x ULN

- Calculated creatinine clearance ≥ 50 mL/min by Cockcroft-Gault formula.

- Left ventricular ejection fraction (LVEF) ≥ 50% by Echocardiogram (ECHO) or multiple
gate acquisition scan (MUGA)

- All study participants must be registered into the mandatory RevAssist program, and be
willing and able to comply with the Requirements of RevAssist.

- Disease-free of prior malignancies for ≥ 5 years with exception of currently treated
basal cell, squamous cell carcinoma of the skin, or carcinoma "in situ" of the cervix
or breast.

- Females of childbearing potential (FCBP) must have a negative serum or urine pregnancy
test with a sensitivity of at least 50 U/mL within 10 to 14 days and again within 24
hours prior to prescribing lenalidomide for Cycle 1 (prescriptions must be filled
within 7 days) and must either commit to continued abstinence from heterosexual
intercourse or begin 2 acceptable methods of birth control, one highly effective
method and one additional effective method at the same time, at least 28 days before
she starts taking lenalidomide. FCBP must also agree to ongoing pregnancy testing.

- Men must agree to use a latex condom during sexual contact with a FCBP even if they
have had a successful vasectomy.

- Ability to understand and the willingness to sign a written informed consent document.

- Able to adhere to the study visit schedule and other protocol requirements.

- Able to take aspirin (81 or ≥ 25 mg) daily as prophylactic anticoagulation. Patients
intolerant to aspirin may use warfarin or low molecular weight heparin (LMWH).
Patients with previous thromboembolic event on lenalidomide or thalidomide may be
started on warfarin or LMWH. Patients already taking warfarin or LMWH do not require
additional aspirin..

- Lactating females must agree not to breast-feed while taking lenalidomide

Exclusion Criteria:

- Any serious medical condition, laboratory abnormality, or psychiatric illness that
would prevent the subject from signing the informed consent form.

- Pregnant or breastfeeding females.

- Any concurrent severe or uncontrolled medical disease which places the subject at
unacceptable risk if he/she were to participate in the study or confounds the ability
to interpret data from the study.

- Use of any other experimental drug or therapy within 28 days of first dose of
amrubicin.

- Known hypersensitivity to thalidomide or lenalidomide.

- The development of erythema nodosum if characterized by a desquamating rash while
taking thalidomide or similar drugs.

- LVEF ≤ 50%.

- Concurrent use of other anti-cancer agents or treatments.

- Known positive for HIV, or infectious hepatitis, type B or C.

- Cranial radiotherapy ≤ 21 days prior to first dose of amrubicin; radiotherapy to all
other areas ≤ 7 days prior to first dose of amrubicin.