Immunological Characterization of Blood of Normal Individuals
| Status: | Recruiting |
|---|---|
| Healthy: | No |
| Age Range: | 18 - Any |
| Updated: | 3/1/2014 |
| Start Date: | March 2011 |
| End Date: | December 2020 |
| Contact: | Pulmonary Clinical Trials Office |
| Email: | Lung.Research@osumc.edu |
| Phone: | 614-293-4978 |
This study seeks to obtain normal samples of blood, saliva and urine to serve as controls
for other approved protocols and for analysis. These include determination of the functional
capabilities of cells, proteins, nucleic acids and microvesicles obtained from peripheral
blood including plasma, serum and cellular fraction from normal individuals. Saliva and
urine will have similar testing which may also be compared to the blood testing results.
The ongoing objective varies from investigation to investigation but in general the
investigational goals are to better understand the immune function of normal volunteers and
compare it to immune function in disease states.
for other approved protocols and for analysis. These include determination of the functional
capabilities of cells, proteins, nucleic acids and microvesicles obtained from peripheral
blood including plasma, serum and cellular fraction from normal individuals. Saliva and
urine will have similar testing which may also be compared to the blood testing results.
The ongoing objective varies from investigation to investigation but in general the
investigational goals are to better understand the immune function of normal volunteers and
compare it to immune function in disease states.
Blood will be obtained via peripheral venipuncture. The blood will be divided into
components (e.g. plasma, serum, microvesicles, and cells) then analyzed. Flow cytometry
will be used to determine cell and microvesicle subsets. RNA will be extracted from the
various components of blood and will be subjected to gene and microRNA profiling to
determine gene expression. DNA will be isolated from the cells to be used in studies
investigating epigenetic regulation of genes identified in the screens. Protein will also be
isolated from the multiple components to examine the impact of genetic regulation by
epigenetic mechanisms or microRNA on protein expression. Peripheral blood mononuclear cells
will be placed in tissue culture for study.
Saliva and urine will be obtained the same day as blood for testing.
components (e.g. plasma, serum, microvesicles, and cells) then analyzed. Flow cytometry
will be used to determine cell and microvesicle subsets. RNA will be extracted from the
various components of blood and will be subjected to gene and microRNA profiling to
determine gene expression. DNA will be isolated from the cells to be used in studies
investigating epigenetic regulation of genes identified in the screens. Protein will also be
isolated from the multiple components to examine the impact of genetic regulation by
epigenetic mechanisms or microRNA on protein expression. Peripheral blood mononuclear cells
will be placed in tissue culture for study.
Saliva and urine will be obtained the same day as blood for testing.
Inclusion Criteria:
- Healthy with no chronic or acute illnesses, or taking medication which in the opinion
of the PI would impact the analyses being conducted.
- At least 18 years or older.
- Are capable of reading, understanding and providing written informed consent.
Exclusion Criteria:
- Non-English speaking.
- Individuals under 18 years old.
- Woman who believe they are or may be pregnant. (Based on self reporting. Pregnancy
testing will not be conducted for the purposes of this study)
- Individuals experiencing acute or chronic illnesses which in the opinion of the PI
would impact the analyses being conducted.
- Currently taking prescription or over the counter medication which in the opinion of
the PI would impact the analyses being conducted.
- Individual has donated blood more than twice in the last week or the amount to be
drawn will exceed the maximum allowance in the last 8 weeks.
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