Relation of Diet to Heart Disease Risk Factors in Children
| Status: | Completed |
|---|---|
| Conditions: | Healthy Studies, Peripheral Vascular Disease, Cardiology |
| Therapuetic Areas: | Cardiology / Vascular Diseases, Other |
| Healthy: | No |
| Age Range: | Any |
| Updated: | 11/18/2012 |
| Start Date: | September 2011 |
| End Date: | July 2012 |
| Contact: | Ronald M Krauss, MD |
| Email: | rkrauss@chori.org |
| Phone: | 510-450-7908 |
Metabolites of dietary phosphatidylcholine- choline and trimethylamine N-oxide (TMAO)- were
recently identified as being associated with myocardial infarction in a case-control study.
The latter TMAO is a gut-microflora-derived choline metabolite that has been shown to be a
potent risk factor for cardiovascular disease (CVD). This pilot study seeks to use
information derived from a dietary questionnaire in children to test the association of
dietary choline intake to plasma levels of TMAO as well as the relationship between plasma
choline levels and components of atherogenic dyslipidemia (increased triglycerides and small
LDL, and reduced HDL cholesterol). An ancillary goal of this study is to build on existing
programs of community outreach to local Oakland/Berkeley minority communities, and to
develop an infrastructure for family-based and community participation in clinical research
across the full age spectrum and among diverse populations. This pilot study will examine
the association of dietary choline intake assessed by food frequency questionnaires to
biomarkers of CVD risk in 40 children (> 7 years of age) and their parents as there is no
information regarding this relationship in children. The results of this pilot study will
form the basis for a proposal to carry out a randomized intervention trial to directly test
the effects of dietary choline intake on plasma TMAO and lipoprotein levels. Ultimately,
better understanding of the relationship between dietary choline intake and CVD risk factors
may facilitate the formulation of appropriate dietary choline recommendations in children
and adults.
From interested participants (children and parents) we will obtain:
1. Informed consent
2. Anthropometric measurements - height, weight, waist and hip circumference, blood
pressure, % body fat by bioimpedance (Tanita scale).
3. Health History Questionnaire
4. Food Frequency Questionnaire
5. Fasting blood sample- We will collect a total of 40 ml of blood (less than 3 ml/kg for
the entire study). The blood samples will be used to measure triglyceride,
LDL-cholesterol, HDL-cholesterol, glucose, insulin, apoA1, apoB, C-reactive protein,
lipoprotein subfraction analysis by ion mobility, DNA for inclusion in our DNA biobank,
choline, TMAO and other metabolites related to heart disease risk.
Standard Blood sampling: Using standard blood collection procedures, blood samples will be
collected from participants after a 12-14 hour fast. The blood will be collected in tubes
containing the following preservative solution: 3.0 gms EDTA (dipotassium), 1.7 mg P-Pack,
0.15 gms gentamycin sulfate, 0.15 gms chloramphenicol, 5.96 mls aprotinin (Sigma A-6279),
and 0.30 gms sodium azide all of which are diluted to 20mls with doubly deionized water.
Plasma is separated by immediate centrifugation at 4°C. Lipid and lipoprotein measurements
are performed and aliquots of plasma are frozen for future analyses.
Inclusion Criteria:
- Children >7 years of age and their parents
Exclusion Criteria:
- Antibiotics
- Pregnant or breastfeeding women
We found this trial at
1
site
747 52nd St
Oakland, California 94609
Oakland, California 94609
(510) 428-3000
Children's Hospital and Research Center Oakland For nearly 100 years, Children's Hospital & Research Center...
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