Oocyte Vitrification for In Vitro Fertilization (IVF) Patients and Women With Fertility Threatening Conditions

Inclusion Criteria:

- Women between the ages of 18 and 37 and who require IVF for a successful pregnancy
will be approached to consent for the study,. They must also have a cycle day 3 FSH
< 10 mIU/ml, estradiol < 70 pg/ml and a baseline follicle count > 10. The BMI must be
between 20 and 32kg/m2. If they do consent, eggs will only be vitrified if 13 or
more mature metaphase II oocytes are collected.

- Women between the ages of 14 and 42 who are seeking treatment for fertility
preservation. In addition they must also meet the following criteria.

- Patient is willing and able to delay any relevant therapy for up to 40 days.

- Have functioning ovaries as determined by blood / ultrasound testing.

- Patient is willing to undergo infectious disease testing for HIV, hepatitis B and C,
syphilis, gonorrhea and Chlamydia.

- The patient must have clearance from their oncologist or medical specialist, if any,
to undergo ovarian stimulation and transvaginal oocyte retrieval.

- The patient must not have a past history of blood clots in the veins or in the lungs.

- The patient must be willing to undergo transvaginal, internal ultrasound examination
and pelvic examination.

Exclusion Criteria:

- Patients with a cycle day 3 FSH > 10 mIU/ml, cycle day 3 estradiol > 70 pg/ml and a
baseline follicle count < 10 will be excluded.

- Patients with a BMI <20 and >32 will be excluded.

- Patients who are not willing or are unable to meet the above mentioned eligibility
requirments.

- Patients with expected low ovarian reserve [evaluated on day 3 by an antral follicle
count of <3 follicles at 2-5 mm diameter and/or an increased concentration of
follicle stimulating hormone (FSH) >15 IU [15] on cycle day 3 ].

- Patients with possible poor quality of oocytes, based on medical history including
previous exposure to chemotherapeutics or radiation.