Optical Imaging of Head and Neck Cancer

The purpose of this study is to determine if optical imaging modalities used at the time of
surgical resection for head and neck squamous cell carcinoma can help delineate normal from
cancerous mucosa. The High resolution microendoscope, developed by our collaborators at Rice
university, can allow for real time visualization of tissue nuclei. The overall aim of this
study is to determine if this device can be used to enhance the accuracy of intraoperative
margin detection during tumor resection for head and neck cancer.

At the time of tumor resection for head and neck squamous cell carcinoma, a wide field
imaging device will be used to identify suspicious areas. The High resolution device will
then image representative areas from the tumor, the tumor margin, and normal mucosa. A
topical dye, proflavin, will be placed on the tissue to enhance the visualization of nuclei
prior to imaging with the HRME device. Following imaging, biopsies of the imaged areas will
be taken and submitted for pathology diagnosis. The images of the biopsies will then be
compared and the device will be evaluated for accuracy of margin detection at the time of
tumor resection.

Inclusion Criteria:

- Biopsy Proven Squamous Cell Carcinoma of the oral cavity, oropharynx, larynx,
hypopharynx

- Must be receiving surgical treatment for their cancer

Exclusion Criteria:

- Presence of medical or psychiatric condition affecting the ability to give informed
consent

- Known allergy to Proflavin

- Pregnant or nursing Females