Autologous Endometrial Coculture (AECCT)

Young women with poor ovarian reserve are commonly given a poor prognosis for IVF success.
Standard culture media for fertilizing oocytes (unfertilized eggs) is commonly used in IVF
which closely resembles tubal fluid. Efforts to create optimal culture conditions for
fertilization of human embryos for women with poor quality embryos and/or repeated
implantation failures have been developed at our center by using AECC. AECC involves placing
a patient's fertilized eggs on top of a layer of cells from her own uterine lining, creating
a more natural environment for embryo development and maximizing the chance for IVF
pregnancy. The investigators will prospectively randomize young patients (≤ age 38 years)
poor ovarian reserve to AECC or no AECC with standard culture media to study pregnancy
outcome.

Inclusion Criteria:

- Healthy women undergoing IVF at CRMI

- Age ≤ 38 years

- Poor ovarian reserve with antral follicle count less than 6 follicles, cycle day 2/3
FSH > 12 ng/ml, AMH < 0.5 ng/ml

Exclusion Criteria:

- Healthy women undergoing IVF with male factor, severe endometriosis, or tubal factor
infertility

- Donor patients

- Contraindications for the use of gonadotropins (i.e. pregnancy, lactation,
undiagnosed vaginal bleeding or ovarian cysts)

- Recent or current medical conditions where the patient is not medically stable to
undergo stimulation or egg retrieval, HIV infection, diabetes, cardiovascular
disease, gastrointestinal, hepatic disease, renal or pulmonary disease.

- Any patient who is not a candidate for IVF