Drug-Drug Interaction Study With Pravastatin and Cyclosporine

Screening Procedures:

Prior to enrollment, subjects will be asked to come to the Clinical and Translational
Science (CTSI) Clinical Research Center (CRC) at San Francisco General Hospital (SFGH). The
study protocol and procedures will be explained in detail, all questions will be answered,
and subjects will be provided with a consent form to sign. The screening visit will be
conducted within 14 days of the first inpatient visit (i.e. first pravastatin with placebo
or pravastatin with cyclosporine dosing day)

Only after subjects have consented to participate in the study will the screening procedures
commence. During the screening visit, vital signs as well as blood sample (10 mL) will be
taken by venipuncture to measure a complete blood count (CBC), electrolytes, blood urea
nitrogen (BUN), creatinine, and liver function tests (LFTs) to screen for anemia and renal
or hepatic insufficiency (see Inclusion/Exclusion Criteria)

Procedures During Main Study:

An equal number of subjects will be randomized, by a computer program, into one of the two
study arms described below. On the first day of the study, subjects will be dosed with
either 100 mg cyclosporine or placebo at 8PM, followed by an overnight fast. On the morning
of Day 2, subjects will be asked to take a second dose of 100 mg cyclosporine or placebo at
8AM. At 9AM of Day 2, subjects will be asked to take 40 mg pravastatin. Plasma samples (5
ml) will be collected at baseline (prior to pravastatin administration), then at 0.25, 0.5,
0.75, 1, 1.5, 2, 2.5, 3, 4, 5, 6, 7, 8, 10, 12 hours post-dose. Whole blood will also be
collected 12 hours post-dose to measure the cyclosporine level.

The procedures during the second visit of the study will be identical to the first, but with
dosing of 100 mg cyclosporine or placebo, opposite of what was dosed at the first study
visit.

Clinical and Laboratory Determinations:

Analytical Methods:

Measurement of pravastatin in plasma will be performed by High Performance Liquid
Chromatography (HPLC) with tandem mass spectrometry (MS/MS), using assays previously
described and validated.

Inclusion Criteria:

- Carrier of one of the three SLCO1B1 polymorphisms that we are studying (SLCO1B1
521TT, 521TC, 521CC)

- Men or women (women must have negative pregnancy test)

- ages 18 to 45 years

- Caucasian

- Healthy, including no renal or hepatic disease, as determined from plasma creatinine
and liver enzyme levels

- No medications currently, except for vitamins and oral contraceptive pills

- Willingness to spend two nights at the San Francisco General Hospital

- Sign informed consent

Exclusion Criteria:

- Under 18 years old

- Unable to confirm Caucasian ancestry

- Pregnant or lactating women (female subjects will have a urine pregnancy test at the
screening visit)

- Prior history of allergic reaction to pravastatin or cyclosporine

- Has a risk of congestive heart failure requiring pharmacologic treatment (medical
history)

- Has prior renal or hepatic dysfunction

- Risk of urinary or gastric retention or narrow-angle glaucoma (by medical history
examination)

- Impaired renal function (e.g. as suggested by abnormal creatinine clearance, eGFR <
60 or serum creatinine > 1.4 mg/dl in females and > 1.5 mg/dl in males) which may
also result from conditions such as cardiovascular collapse (shock), acute myocardial
infarction (heart attack), and septicemia, abnormal heart rhythms (tachyarrhythmias;
heart beat > 100 beats per minute)

- Impaired hepatic function (> 1.5 times the upper limit of normal)

- Evidence of anemia (hemoglobin <10g)

- Taking medications other than vitamins that could confound study results

- They do not provide informed consent to participate in the study