Rapid Oral Test for Therapeutic Response in HIV/AIDS Patients
| Status: | Completed |
|---|---|
| Conditions: | HIV / AIDS |
| Therapuetic Areas: | Immunology / Infectious Diseases |
| Healthy: | No |
| Age Range: | Any |
| Updated: | 2/17/2018 |
| Start Date: | October 2011 |
| End Date: | October 2013 |
This study has been designed to evaluate a new oral test for therapeutic monitoring of
HIV/AIDS patients that are receiving the combination Anti-Retroviral Therapy (cART). The test
will measure saliva-based Stress Response Profiling(SRP) biomarkers using laboratory assays.
Results of the test will show if HIV/AIDS patients successfully responded to cART.
Preliminary studies showed that SRP biomarkers were strongly increased in cART-unresponsive
AIDS patients. However, the diagnostic accuracy of the oral test, patients will be recruited
to donate saliva: AIDS patients responsive or unresponsive to cART, and controls (acute or
early HIV patients, and HIV-negative patients with hepatitis).
The saliva samples will be used to measure SRP biomarker concentrations. Results will show
whether the biomarker measurements provide accurate and specific diagnostics for ART
response.
HIV/AIDS patients that are receiving the combination Anti-Retroviral Therapy (cART). The test
will measure saliva-based Stress Response Profiling(SRP) biomarkers using laboratory assays.
Results of the test will show if HIV/AIDS patients successfully responded to cART.
Preliminary studies showed that SRP biomarkers were strongly increased in cART-unresponsive
AIDS patients. However, the diagnostic accuracy of the oral test, patients will be recruited
to donate saliva: AIDS patients responsive or unresponsive to cART, and controls (acute or
early HIV patients, and HIV-negative patients with hepatitis).
The saliva samples will be used to measure SRP biomarker concentrations. Results will show
whether the biomarker measurements provide accurate and specific diagnostics for ART
response.
Inclusion Criteria:
Group 1, cART-unresponsive
- Documented HIV-positive before obtaining AIDS diagnosis
- cART for 18 months or longer
- consistent lab tests in previous 3-6 months: < or equal to 200 CD4 T cells/mm3, viral
load >5000 HIV RNA copies/ml
Group 2, cART-responsive
- Documented HIV-positive before obtaining AIDS diagnosis
- cART for 18 months or longer
- consistent lab tests in previous 3-6 months: > or equal to 350 CD4 T cells/mm3, viral
load < or equal to 50 HIV RNA copies/ml
Group 3, acute/early HIV
Acute HIV:
- Signs and symptoms of acute retroviral syndrome in a person with either a negative or
evolving antibody response (western blot or ELISA) in presence of a positive HIV
p24gag antigen or HIV-1 proviral DNA PCR or positive HIV-1 RNA
Early HIV:
- A positive antibody response (western blot or ELISA) with a documented negative
serological test or plasma HIV-1 RNA within the past 12 months or a positive ELISA and
a negative de-tuned ELISA within 30 days of a positive ELISA specimen in an untreated
person with no clinical or immunological evidence of advanced HIV disease (CD4 count
>200 cells/mm3 or >14%)
Group 4, HIV-negative Hepatitis-positive
- HIV test negative (western blot, ELISA or viral load)
- Positive Hepatitis B surface antigen and detectable hepatitis B viral load (HCV B
RNA), treated or not OR Positive Hepatitis C antibodies and detectable hepatitis C
viral load (HCV C RNA), treated or not
Exclusion Criteria:
- Pregnant or lactating women
- Non-adherent patients
- Any conditions which will affect saliva production: e.g. Sjogren's syndrome or chronic
mouth dryness
- Cytotoxic chemotherapy, interferon treatment, or radiation therapy within the
preceding 3 weeks
- Active alcohol or substance abuse (narcotics or other controlled substances) within 6
months prior to the saliva sampling
- Significant psychiatric illness that in the opinion of the principle investigator
might interfere with making an informed decision
- Incapable to give informed consent
We found this trial at
1
site
Click here to add this to my saved trials
