Detecting Respiratory Viruses in Upper and Lower Respiratory Tract Samples
| Status: | Terminated |
|---|---|
| Conditions: | Infectious Disease, Pulmonary |
| Therapuetic Areas: | Immunology / Infectious Diseases, Pulmonary / Respiratory Diseases |
| Healthy: | No |
| Age Range: | 12 - 99 |
| Updated: | 5/5/2018 |
| Start Date: | September 1, 2012 |
| End Date: | May 1, 2018 |
Detection of Respiratory Viruses in Upper and Lower Respiratory Tract Specimens Using a Rapid Multiplex PCR
Background:
- Bronchoalveolar lavage (BAL) is a procedure where a tube is passed through the mouth or
nose into the lungs. Fluid is squirted through the tube into a part of the lung and then
collected for examination. It is used to detect respiratory viruses. BAL is a relatively
invasive procedure, and researchers want to test the accuracy of other procedures that do not
involve collecting fluid from the lungs. The nasopharynx is the area of the upper throat that
lies behind the nose. Researchers want to see if a swab taken from this area is as accurate
as a BAL sample.
Objectives:
- To see if a nasopharynx swab can be used to detect respiratory viruses as well as BAL
samples.
Eligibility:
- Individuals at least 12 years of age who will have a bronchoscopy to collect a BAL sample
to test for respiratory viruses.
Design:
- Participants will be screened with a physical exam and medical history.
- Participants will have a BAL sample collected.
- Participants will then have a nasopharynx swab. The swab will be inserted into the nose,
left in place for up to 10 seconds, and then removed
- Bronchoalveolar lavage (BAL) is a procedure where a tube is passed through the mouth or
nose into the lungs. Fluid is squirted through the tube into a part of the lung and then
collected for examination. It is used to detect respiratory viruses. BAL is a relatively
invasive procedure, and researchers want to test the accuracy of other procedures that do not
involve collecting fluid from the lungs. The nasopharynx is the area of the upper throat that
lies behind the nose. Researchers want to see if a swab taken from this area is as accurate
as a BAL sample.
Objectives:
- To see if a nasopharynx swab can be used to detect respiratory viruses as well as BAL
samples.
Eligibility:
- Individuals at least 12 years of age who will have a bronchoscopy to collect a BAL sample
to test for respiratory viruses.
Design:
- Participants will be screened with a physical exam and medical history.
- Participants will have a BAL sample collected.
- Participants will then have a nasopharynx swab. The swab will be inserted into the nose,
left in place for up to 10 seconds, and then removed
Bronchoalveolar lavage (BAL) specimens can be used for the detection of respiratory viruses.
Although bronchoscopy is relatively safe, it still has inherent risks to the subject.
Nasopharyngeal (NP) swab specimens are easy to collect and are minimally invasive for the
subject. We will use a rapid multiplex polymerase chain reaction (PCR) to detect respiratory
viruses in paired BAL and NP specimens to determine if either specimen is more likely to be
positive. The study population will include all patients undergoing bronchoscopy for clinical
indications at the Clinical Center. This is a prospective observational study.
Although bronchoscopy is relatively safe, it still has inherent risks to the subject.
Nasopharyngeal (NP) swab specimens are easy to collect and are minimally invasive for the
subject. We will use a rapid multiplex polymerase chain reaction (PCR) to detect respiratory
viruses in paired BAL and NP specimens to determine if either specimen is more likely to be
positive. The study population will include all patients undergoing bronchoscopy for clinical
indications at the Clinical Center. This is a prospective observational study.
- INCLUSION CRITERIA:
- Age 12 years or above
- Scheduled for clinically indicated bronchoscopy for the collection of a BAL specimen
and will have respiratory virus PCR ordered on the BAL specimen as part of their
ongoing care at the NIH Clinical Center.
- Must agree to allow the storage of their samples (BAL and NP) for use in future
research.
EXCLUSION CRITERIA:
We found this trial at
1
site
9000 Rockville Pike
Bethesda, Maryland 20892
Bethesda, Maryland 20892
Phone: 800-411-1222
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