Severe Asthma Research Program

The mission of the SARP is to improve the understanding of severe asthma to develop better
treatments. The SARP will gain a better understanding of asthma and its endotypes, in
children and adults, by defining the disease at the molecular and cellular levels in the
context of the temporal phenotypic expression of the disease. To this end, the SARP
investigators will utilize both mechanistic and evoked phenotype approaches to: 1)
characterize developmental molecular, cellular and physiologic phenotypes in children and
adults with mild to severe asthma, and 2) to further elucidate the evolving pathobiology and
pathogenesis of severe asthma and its sub-phenotypes and 3) compare these features over time.
This approach involves a shared longitudinal protocol conducted across all participating
centers which includes common information on all SARP participants. Additionally, the SARP
investigators have each identified mechanistic research questions to be included in the
shared longitudinal protocol. Together, these longitudinal and mechanistic approaches will
enable prediction of phenotype stability/fluctuation and pharmacologic responses and
identification of novel, disease-modifying targets for treatment.

Asthmatic Patients:

Inclusion Criteria:

1. Physician diagnosis of asthma,

2. Age 6 years and older

3. Evidence of historical reversibility, including either:

- FEV1 bronchodilator reversibility ≥ 12%, or

- Airway hyperresponsiveness reflected by a methacholine PC20 ≤16 mg/mL.

Exclusion Criteria:

1. Pregnancy during the characterization phase,

2. Current smoking,

3. Smoking history > 10 pack years if ≥30 years of age, or smoking history > 5 pack years
if <30 years of age (Note: if a subject has a smoking history, no smoking within the
past year),

4. Other chronic pulmonary disorders associated with asthma-like symptoms, including (but
not limited to) cystic fibrosis, chronic obstructive pulmonary disease, chronic
bronchitis, vocal cord dysfunction (that is the sole cause of respiratory symptoms and
at the PI's discretion), severe scoliosis or chest wall deformities that affect lung
function, or congenital disorders of the lungs or airways,

5. History of premature birth before 35 weeks gestation,

6. Unwillingness to receive an intramuscular triamcinolone acetonide injection,

7. Evidence that the participant or family may be unreliable or poorly adherent to their
asthma treatment or study procedures,

8. Planning to relocate from the clinical center area before study completion,

9. Any other criteria that place the subject at unnecessary risk according to the
judgment of the Principal Investigator and/or attending physician(s) of record, or

10. Currently participating in an investigational drug trial for asthma therapies.

Healthy Controls:

Inclusion criteria: Healthy subjects between the age of 18 and 65 years. At least 3 of the
7 subjects per center should be aged 35 years or older.

Exclusion criteria

1. History of chronic diseases that affect the lungs,

2. A history suggestive of allergic rhinitis, eczema or chronic sinusitis,

3. An improvement in FEV1 of more than 12% following 4 puffs of albuterol,

4. Smoking history > 10 pack years if ≥30 years of age, or smoking history > 5 pack years
if <30 years of age, or any smoking within the past year,

5. Respiratory tract infection within the past 4 weeks,

6. Pregnancy,

7. History of premature birth (<35 weeks).