Panobinostat and Ruxolitinib In MyElofibrosis (PRIME Trial)

Phase I/II open label, single institution, combination therapy trial of induction Ruxolitinib
followed by combination with Panobinostat in dose escalation cohorts with a primary endpoint
of determining the safety and tolerability of combination therapy in patients with
myelofibrosis (MF) in chronic and accelerated phase. A 3+3standard dose escalation scheme
will be employed and the occurrence of dose limiting toxicities (DLTs) will be captured and
the occurrence of such events will determine dose cohort escalation by predetermined and
established rules. In addition to establishing the DLTs, maximally tolerated dose (MTD), and
recommended phase II dose (RPTD) in the phase I portion of this trial, exploratory biomarkers
will be evaluated within phase I as well. Pharmacodynamics and exploratory genetic and
epigenetic biomarkers will be explored as predictors of response to therapy. The RPTD cohort
will be expanded to incorporate a total of 22 patients, including 6 from phase I, in order to
assess clinical response as assessed by International Working Group for Myelofibrosis
Research and Treatment (IWG-MRT) as a primary endpoint for the phase II portion of this
trial.

Inclusion Criteria:

- Male or female patients aged ≥ 18 years old

- Ability to provide written informed consent obtained prior to participation in the
study and any related procedures being performed

- Intermediate-2 and higher by IWG-MRT Post PV/ET MF and PMF patients either in

1. Chronic Phase (MF-CP)

2. Accelerated Phase (MF-AP)

- Patients must meet the following laboratory criteria:

1. ANC ≥ .750 x 109/L

2. Platelets ≥ 75 x 109/L

3. Creatinine ≤ 1.5 x ULN,

4. AST and ALT ≤ 2.5 x ULN

5. Serum bilirubin ≤ 1.5 x ULN (unless Gilbert's syndrome and evidence of hemolysis)

6. Serum potassium ≥ LLN

7. Total serum calcium [corrected for serum albumin] or ionized calcium ≥LLN,

8. Serum magnesium ≥ LLN

9. Serum phosphorus ≥ LLN

10. Free T4 within normal limits

- ECOG Performance Status of ≤ 3

- Any prior therapy with JAK2-TKI, hypomethylating agents, HDACI, mTORi, or iMiDs is
allowed as long as it is greater than 3 weeks since last dose of administration and in
the case of a JAK2-TKI or HDACI that discontinuation was not due to non-hematologic
drug toxicity. An exception to this criteria are patients currently on at least 10mg
BID of ruxolitinib for greater than 3 months and who have not shown an optimal
response (i.e. without 50% reduction in palpable splenomegaly or 50% reduction in
symptom burden). With a reduction of ruxolitinib to 10mg BID these patients may enter
onto the study without stopping ruxolitinib

Exclusion Criteria:

- Patients who will need valproic acid for any medical condition during the study or
within 5 days prior to first PANOBINOSTAT treatment.

- Impaired cardiac function or clinically significant cardiac diseases, including any
one of the following:

1. With permanent cardiac pacemaker

2. Resting bradycardia defined as <50 beats per minute

3. QTcF >450 msec on screening ECG

4. Complete Left bundle branch block, bifascicular block

5. Any clinically significant ST segment and/or T-wave abnormalities

6. Presence of unstable atrial fibrillation (ventricular response rate >100 bpm).
Patients with stable atrial fibrillation can be enrolled provided they do not
meet other cardiac exclusion criteria.

7. Symptomatic congestive heart failure (NYHA class III-IV)

- Impairment of GI function or GI disease that may significantly alter the absorption of
PANOBINOSTAT or RUXOLITINIB

- Other concurrent severe and/or uncontrolled medical conditions (e.g., uncontrolled
diabetes or active or uncontrolled infection) including abnormal laboratory values,
that could cause unacceptable safety risks or compromise compliance with the protocol

- Patients using medications that have a relative risk of prolonging the QT interval or
inducing torsade de pointes if treatment cannot be discontinued or switched to a
different medication prior to starting study drug

- Concomitant use of CYP3A4 inhibitors

- Patients who have received targeted agents within 2 weeks or within 5 half-lives of
the agent and active metabolites (whichever is longer) and who have not recovered from
side effects of those therapies.

- Chemotherapy within 3 weeks prior to screening are excluded (other than hydroxyurea at
stable doses and will be discontinued 24 hours prior to starting study drug).

- Patients with an active bleeding tendency or are receiving any treatment with
therapeutic doses of sodium warfarin (Coumadin®) or coumadin derivatives. Patients
will be allowed to enter study on aspirin at doses of 81mg/d.

- Patients who have undergone major surgery ≤ 4 weeks prior to starting study drug or
who have not recovered from side effects of such therapy

- Women who are pregnant or breast-feeding or women of childbearing potential (WOCBP)
not using an effective method of birth control. WOCBP are defined as sexually mature
women who have not undergone a hysterectomy or who have not been naturally
postmenopausal for at least 12 consecutive months (i.e., who has had menses any time
in the preceding 12 consecutive months). Women of childbearing potential must have a
negative serum pregnancy test within 24hrs of receiving the first dose of study
medication.

- Male patients whose sexual partners are WOCBP not using effective birth control

- Patients with a prior malignancy within the last 5 years (except for basal or squamous
cell carcinoma, or in situ cancer of the cervix)

- Disease associated with secondary MF such as metastatic carcinoma, lymphoma,
myelodysplasia, hairy cell leukemia, mast cell disease or acute leukemia (including M7
disease or acute panmyelosis with MF)