Comparison Of Molecular Targets In Mild To Severe Asthmatics And Healthy Subjects

Inclusion criteria:

- Male or female subjects aged between 18-60 years inclusive at screening.

- A female subject of childbearing potential must be using effective contraceptive

- measures described in protocol for at least one month prior to Screening and should
continue using the same contraceptive measure during the study until completion of
follow-up procedures. Non child-bearing potential is defined as pre-menopausal
females with documented (medical report verification) hysterectomy or surgical
sterilisation, or post-menopausal women who have been amenorrheic for more than 1
year and having estradiol and FSH levels consistent with menopause.). Other methods
include male partner who is sterile prior to female subject's entry into study and is
the sole sexual partner for that female subject.

- Atopic or non-atopic subjects

- Able to comprehend the nature of this research protocol and other relevant medical
information and the ability to give written informed consent prior to participation
in the study.

- Able to comply with the requirements and restrictions listed in the consent form.

- Available to complete the study and all measurements.

- Read, comprehend, and write English at a sufficient level to complete study related
materials.

Additional inclusion criteria for the steroid naïve intermittent asthmatics - Cohort A

- Have a pre-history of a physician's diagnosis of asthma, with exclusion of other
significant pulmonary

- No significant disease other than asthma.

- No history of steroid myopathy.

- No history of recent exposure to live vaccine in the past 4 weeks and no intention to
undergo live vaccination during the prednisolone trial or during the month following
the trial.

- Intermittent asthma with FEV1 ≥ 80% predicted.

- Positive beta agonist reversibility as shown by greater than 12% improvement on
FEV1or greater than 200ml improvement in FEV1 within 30 minutes following the
administration of Albuterol Inhalation Aerosol OR a positive PC20 to Methacholine
Challenge smaller than 16 mcg.

- Not currently taking inhaled steroids (ICS), and has not taken ICS for at least 6
months prior to screening.

- A non-smoker (as demonstrated by a negative urine cotinine) for at least the past 12
months with a pack history ≤5 pack years.

Additional inclusion criteria for the mild to moderate persistent asthmatics on regular
inhaled steroids - Cohort B

- Have a pre-history of a physician's diagnosis of asthma, with exclusion of other
significant pulmonary diseases

- No significant disease other than asthma.

- No history of steroid myopathy.

- No history of recent exposure to live vaccine in the past 4 weeks and no intention to
undergo live vaccination during the prednisolone trial or during the month following
the trial.

- Mild to moderate persistent asthmatic with FEV1 ≥ 80% predicted

- Asthma symptoms ranging from daily to less than once a day

- Positive beta agonist reversibility as shown by greater than 12% improvement on
FEV1or greater than 200ml improvement in FEV1 within 30 minutes following the
administration of Albuterol Inhalation Aerosol OR a positive PC20 to Methacholine
Challenge smaller than 16 mcg.

- On regular inhaled steroid treatment (from 200-500 mcg FP daily or equivalent). Short
and long acting beta-2 agonists, anti-cholinergies, and Leukotriene receptor
antagonists are allowed as concurrent medication.

- A non-smoker (as demonstrated by a negative urine cotinine) for at least the past 12
months with a pack history ≤5 pack years.

Additional inclusion criteria for the severe persistent asthmatics on maximal therapy;
regular oral steroids - Cohort C

- Have a pre-history of a physician's diagnosis of asthma, with exclusion of other
significant pulmonary diseases

- No significant disease other than asthma.

- No history of steroid myopathy.

- No history of recent exposure to live vaccine in the past 4 weeks and no intention to
undergo live vaccination during the prednisolone trial or during the month following
the trial.

- Severe persistent asthmatic

- Subjects should have at least one (if on oral steroids) or two (if only on inhaled
steroids) of the following indices:

1. FEV1 <80% (post bronchodilator) and FEV1/FVC ratio <70% predicted;

2. Daily symptoms ± nocturnal symptoms as recorded in diary cards during run-in;

3. severe exacerbations (as defined FACET study Tattersfield 1999) of ≥ twice a
year in at least one of the last two years, as recorded in clinical records

- Positive beta agonist reversibility as shown by greater than 12% improvement on FEV1
or greater than 200ml improvement in FEV1 within 30 minutes following the
administration of Albuterol Inhalation Aerosol OR a positive PC20 to Methacholine
Challenge smaller than 16 mcg.

- High dose inhaled steroids (≥ to 1000 mcg FP daily or equivalent), oral steroids of ≤
20mg prednisolone a day or equivalent.

- A non-smoker (as demonstrated by a negative urine cotinine) for at least the past 12
months with a pack history ≤5 pack years.

Additional inclusion criteria for the currently smoking, mild to moderate persistent
asthmatics on maximal therapy; regular oral steroids - Cohort D

- Have a pre-history of a physician's diagnosis of asthma, with exclusion of other
significant pulmonary

- No significant disease other than asthma.

- No history of steroid myopathy.

- No history of recent exposure to live vaccine in the past 4 weeks and no intention to
undergo live vaccination during the prednisolone trial or during the month following
the trial.

- Smokers (as demonstrated by a positive urine cotinine) with a pack history >5 pack
years

- Mild to moderate persistent asthmatic with FEV1 ≥ 80% predicted

- Asthma symptoms less than once a day

- Positive beta agonist reversibility as shown by greater than 12% improvement on
FEV1or greater than 200ml improvement in FEV1 within 30 minutes following the
administration of Albuterol Inhalation Aerosol OR a positive PC20 to Methacholine
Challenge smaller than 16mcg.

- On regular inhaled steroid treatment (200-500 mcg FP daily or equivalent). (NB:

short and long acting beta-2 agonists, anti-cholinergies, and Leukotriene receptor
antagonists are allowed as concurrent medication.) Additional inclusion criteria for
healthy volunteers, non asthmatic, non smokers - Cohort E Healthy; defined as individuals
who are free of significant cardiovascular, pulmonary (e.g. tuberculosis),
gastrointestinal, hepatic, endocrine, renal, haematological, neurological and psychiatric
disease as determined by medical history, physical examination and clinical
chemistry/haematology/urinalysis investigation.

• A non-smoker (as demonstrated by a negative urine cotinine) for at least the past 12
months with a pack history ≤5 pack years.

Exclusion criteria:

- As a result of medical interview, physical examination or screening investigation the
physician responsible considers the subject unfit for the study.

- The subject has a history of drug or other allergy, which, in the opinion of the
responsible physician, contra-indicates their participation.

- Subject is female who is pregnant or lactating.

- Having participated within 30 days or 5 half-lives in a study receiving an
investigational drug.

- Having participated within 30 days in a study with an invasive procedure.

- Donation of a 500 mL of blood within the previous 56 days or intention to donate
within 56 days of the end of the last bronchoscopy.

- Evidence of recent infection that would preclude participation in the steroid trial
in the judgement of the study physician. The subject may be deferred to later
participation. History of abnormal bruising or bleeding.

- History of alcohol or drug abuse within five years.

- Positive urine test for drugs of abuse including alcohol at screen.

- Abnormal (clinically significant) clinical laboratory test results.

- Medical history of cirrhosis, hepatitis C or hepatitis B or HIV

- Doing night-shift work that will change pattern within at least 5 days prior to study
start through the first follow up visit for Cohorts A through D. This does not
include the follow up visits at 6 and 12 months.

- Female subjects who are unwilling or unable to use an appropriate method of
contraception

- Those who, in the opinion of the investigator, have a risk of non-compliance with
study procedures.

- Concomitant medications that may interfere with study procedures or evaluations.

- History of hypersensitivity to any of the following medications: Lidocaine, Fentanyl,
Versed, Demerol, Midazolam, Epinephrine, Flumanzenil and Naloxon.

- History of hypersensitivity to bronchodilator (such as Albuterol)

- ALL WOMEN OF CHILD BEARING POTENTIAL WHO DO NOT WISH TO USE PROTOCOL APPROVED METHODS
OF CONTRACEPTION WILL BE EXCLUDED.

Additional exclusion criteria for the steroid naïve intermittent asthmatics - Cohort A

- Subject has changed asthma medication within 1 month prior to screening.

- Subject had an asthma exacerbation in the previous month.

- Known sensitivity or allergy to prednisolone.

- History of tuberculosis, glaucoma, epilepsy, severe affective disorder or peptic
ulceration.

- Current use or use within the previous 1 month of oral corticosteroids.

- Current use of any asthma medication except short acting inhaled β2 agonists

- Current use of Methotrexate, cyclosporin, PDE inhibitors, azathioprine or other
immunosuppressive agents, except steroids.

Additional exclusion criteria for the mild to moderate persistent asthmatics on regular
inhaled steroids - Cohort B

- Subject has changed asthma medication within 1 month prior to screening.

- Subject had an asthma exacerbation in the previous month.

- Known sensitivity or allergy to prednisolone.

- History of tuberculosis, glaucoma, epilepsy, severe affective disorder or peptic
ulceration.

- Current use or use within the previous 1 month of oral corticosteroids.

- Current use of Methotrexate, cyclosporin, PDE inhibitors, azathioprine or other
immunosuppressive agents, except steroids.

Additional exclusion criteria for the severe persistent asthmatics on maximal therapy;
regular oral steroids - Cohort C

- Subject has changed asthma medication within 1 month prior to screening.

- Subject had an asthma exacerbation in the previous month.

- Known sensitivity or allergy to prednisolone.

- History of tuberculosis, glaucoma, epilepsy, severe affective disorder or peptic
ulceration.

- Current use or use within the previous 1 month of oral prednisolone or equivalent of
greater than 20mg daily.

- Current use of Methotrexate, cyclosporin, PDE inhibitors, azathioprine or other
immunosuppressive agents, except steroids.

- Subjects should avoid any medications that in the opinion of the physician might
interfere with either the safety of the subject or the interpretation of the results
(e.g.

anti-inflammatory drugs)

Additional exclusion criteria for the currently smoking, mild to moderate persistent
currently smoking asthmatics on regular inhaled steroids - Cohort D

- Subject has changed asthma medication within 1 month prior to screening.

- Subject had an asthma exacerbation in the previous month.

- Known sensitivity or allergy to prednisolone.

- History of tuberculosis, glaucoma, epilepsy, severe affective disorder or peptic
ulceration.

- Current use or use within the previous 1 month of oral corticosteroids.

- Current use of Methotrexate, cyclosporin, PDE inhibitors, azathioprine or other
immunosuppressive agents, except steroids.

Additional exclusion criteria for the healthy volunteers, non asthmatic non smokers -
Cohort E The subject has any history of breathing problems in adult life (i.e. history of
asthmatic symptoms). Screening lung function tests (FEV1) will be performed to confirm
normal lung function parameters (≥80% predicted).