Predictive Medicine Research. (PRE MED)

Introduction:

As a person ages their chance of developing certain diseases such as hardening of the
arteries (atherosclerosis), diabetes, depression, osteoporosis and memory loss (Alzheimer’s
disease) increases. One of the theories that might explain why age increases the risk of
developing these diseases involves a concept called oxidative stress. Oxidative stress is a
process that occurs inside the cells in our body. All the cells in our body are constantly
undergoing various biochemical reactions, which are important to the cell’s life cycle.
Byproducts of these biochemical reactions are molecules called free radicals or
pro-oxidants. Pro-oxidants can be very damaging to the cell and lead to cell death. Cells
also have molecules called antioxidants, which neutralize the pro-oxidants and protect the
cell so it can continue functioning normally. Oxidative stress occurs when there is an
imbalance in the pro-oxidants as compared with the antioxidants. Other studies have shown
that diseases such as hardening of the arteries, depression, osteoporosis and Alzheimer’s
are associated with elevated levels of free radicals or pro-oxidants inside the cells. It is
now possible to measure the levels of pro-oxidants in the cells using blood tests.

Objectives:

The purpose of this study is to determine how reliable and reproducible these measurements
are and also to define a “normal” value or range of values in healthy people. It is our hope
that after we are able to define the normal range of values for oxidative stress we can then
look at people with the aforementioned diseases and measure their levels of oxidative stress
looking for a correlation between the two. If we can show a direct correlation between level
of oxidative stress and these diseases we can begin trying to develop medicines that can
target this area and hopefully prevent or lessen the degree of these diseases.

Tests and Evaluations

In this study we are looking for healthy participants age 30 and older who will undergo
blood tests that will allow us to measure markers of oxidative stress. Participants will
also undergo tests specifically designed to look for evidence of early multi-organ disease.
These exams will include an evaluation of lung function (spirometry test), exercise capacity
(using a stationary bike), bone density scan looking for osteoporosis, ultrasound of the
artery of the neck (carotid) and artery of the arm (brachial), nerve conduction study,
bio-impedance (Body fact composition) and several questionnaires to assess memory and mood.
The participants require one screening visit and a follow up visit to undergo the tests.

For more information or patient referral call Johnson Periera 404-275-0034, Muhammad Amer
404-805-4771 or Salman Sher 404-587-2667

Inclusion Criteria:

- Ages 30-90.

- Healthy normal performance status.

- LDL cholesterol level <120 mg/dl

- Fasting blood glucose <99 mg/dL

- Blood pressure <135 mmHg systolic and <85 mmHg diastolic

- Non-smoker for at least 5 years

- BMI >19 and <26; stable body weight

- Free of neurological and psychological (Axis I) disorders for at least three years.

Exclusion Criteria:

· Any oral antioxidants in the previous 2 weeks.

- On lipid lowering medication in the past 8 weeks.

- History of substance abuse.

- Current neoplasm or history of neoplasia except basal cell cancer.

- Any heart disease except for corrected congenital ht disease. History of neurological
disease.

- History of chronic GI disorders.

- History of Rheumatologic disorders.

- Known endocrine disorders.

- History of Chronic Pulmonary diseases eg. COPD

- Acute illness such as infection in the previous 4 weeks.

- Creatinine > 2

- Liver enzymes >1.5X normal.

- Anemia (Hb < 12.5) or chronic hematological disorder.

- Physical limitation and sedentary status for any reason.

- LDL cholesterol>120.

- Fasting Glucose>99.

- BP >135/85 on 3 repeated measures.

- Current smoker or smoker in the last 5 years.

- History of depression.

- Inability to give informed consent.

- Age <30