Microarray Expression Profiling to Identify Stereotypic mRNA Profiles for Preterm Delivery in Order to Unravel the Biological Mechanisms
| Status: | Completed |
|---|---|
| Conditions: | Women's Studies |
| Therapuetic Areas: | Reproductive |
| Healthy: | No |
| Age Range: | 15 - Any |
| Updated: | 1/5/2019 |
| Start Date: | December 21, 1999 |
Microarray Expression Profiling to Identify Stereotypic mRNA Profiles in Human Parturition
The understanding of the biological mechanisms underlying preterm birth is very limited,
making prevention of preterm birth difficult. The incidence of preterm birth worldwide varies
between 6% to 11% in singleton pregnancies and 64-93% of preterm deliveries occur after the
spontaneous onset of labor (preterm labor) (1). The risk factors associated with preterm
birth include demographic variables such as ethnic group, past obstetric history, and
complications of the current pregnancy such as infection and fetal congenital anomalies. The
current study aims to investigate the basic mechanisms of preterm labor by systematically
cataloging the changes in expression levels of all expressed genes whose sequences are
available. The goals will be accomplished by using microarray technology followed by
quantitative real-time PCR, in situ PCR, in situ hybridization and proteomics.
making prevention of preterm birth difficult. The incidence of preterm birth worldwide varies
between 6% to 11% in singleton pregnancies and 64-93% of preterm deliveries occur after the
spontaneous onset of labor (preterm labor) (1). The risk factors associated with preterm
birth include demographic variables such as ethnic group, past obstetric history, and
complications of the current pregnancy such as infection and fetal congenital anomalies. The
current study aims to investigate the basic mechanisms of preterm labor by systematically
cataloging the changes in expression levels of all expressed genes whose sequences are
available. The goals will be accomplished by using microarray technology followed by
quantitative real-time PCR, in situ PCR, in situ hybridization and proteomics.
The understanding of the biological mechanisms underlying preterm birth is very limited,
making prevention of preterm birth difficult. The incidence of preterm birth worldwide varies
between 6%-11% in singleton pregnancies, and 64-93% of preterm deliveries occur after the
spontaneous onset of labor (preterm labor) (1). The risk factors associated with preterm
birth include demographic variables such as ethnic group, past obstetric history, and
complications of the current pregnancy such as infection and fetal congenital anomalies. The
current study aims to investigate the basic mechanisms of preterm labor by systematically
cataloging the changes in expression levels of all expressed genes whose sequences are
available. The goals will be accomplished by using microarray technology followed by
subsequent confirmative or complementary analyses.
making prevention of preterm birth difficult. The incidence of preterm birth worldwide varies
between 6%-11% in singleton pregnancies, and 64-93% of preterm deliveries occur after the
spontaneous onset of labor (preterm labor) (1). The risk factors associated with preterm
birth include demographic variables such as ethnic group, past obstetric history, and
complications of the current pregnancy such as infection and fetal congenital anomalies. The
current study aims to investigate the basic mechanisms of preterm labor by systematically
cataloging the changes in expression levels of all expressed genes whose sequences are
available. The goals will be accomplished by using microarray technology followed by
subsequent confirmative or complementary analyses.
- INCLUSION CRITERIA:
Consecutive patients admitted with the following diagnoses from four different ethnic
groups (Hispanic, African American, Asian, and Caucasian):
1. Preterm labor with intact membranes and with
1. acute inflammation;
2. chronic villitis;
3. vascular pathology;
4. no identifiable lesions.
2. Preterm delivery without labor because of the following reasons:
1. pre-eclampsia;
2. abruptio placentae;
3. fetal anomalies;
4. Other complications (e.g. automobile accidents) that necessitate immediate
delivery.
3. PROM leading to preterm delivery and with
1. acute inflammation;
2. chronic villitis;
3. vascular pathology;
4. no identifiable lesions.
4. Term delivery without labor and no identifiable lesions.
5. Term delivery in spontaneous labor and no identifiable lesions.
6. Term delivery with chorioamnionitis.
7. Term delivery with failed labor leading to ceasarean section.
EXCLUSION CRITERIA:
1. Refusal of written informed consent
2. Fetal or maternal conditions mandating immediate delivery (i.e. fetal distress,
significant hemorrhage, etc.)
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