A Study of the Effects of PEITC on Oral Cells With Mutant p53
| Status: | Completed |
|---|---|
| Conditions: | Cancer, Cancer |
| Therapuetic Areas: | Oncology |
| Healthy: | No |
| Age Range: | 20 - 65 |
| Updated: | 3/16/2015 |
| Start Date: | February 2012 |
| End Date: | April 2014 |
| Contact: | Daniela A Iliescu |
| Email: | dai4@georgetown.edu |
| Phone: | (202) 687-2295 |
The aim of this clinical trial is to examine the effects of phenethyl isothiocyanate
(PEITC), a compound derived from cruciferous vegetables, on oral cells with mutant p53. The
p53 protein's normal (wild-type) function within cells is to act as a tumor suppressor, or
anti-cancer protein. When mutated, the p53 protein not only no longer executes the
functions of a tumor suppressor, and it can gain functions as a pro-cancer protein.22 The
proposed clinical trial will utilize oral cells collected from subjects who are heavy
smokers. Since the subjects are regularly exposed to mutagenic chemicals it is the thought
that their oral cells will contain measurable levels of mutant p53. The participants will
consume watercress juice, which is rich in PEITC (See Figure 1). Previous studies in the
laboratory showed PEITC selectively depletes mutant p53, and not wild type p53, in cell
culture. Our hypothesis is that the ITC in the watercress juice will lead to the depletion
of mutant p53 within the oral cells. This depletion could lead to a possible clinical
application of this compound, such as chemopreventives or oncologic treatments of
individuals with oral cancers.
(PEITC), a compound derived from cruciferous vegetables, on oral cells with mutant p53. The
p53 protein's normal (wild-type) function within cells is to act as a tumor suppressor, or
anti-cancer protein. When mutated, the p53 protein not only no longer executes the
functions of a tumor suppressor, and it can gain functions as a pro-cancer protein.22 The
proposed clinical trial will utilize oral cells collected from subjects who are heavy
smokers. Since the subjects are regularly exposed to mutagenic chemicals it is the thought
that their oral cells will contain measurable levels of mutant p53. The participants will
consume watercress juice, which is rich in PEITC (See Figure 1). Previous studies in the
laboratory showed PEITC selectively depletes mutant p53, and not wild type p53, in cell
culture. Our hypothesis is that the ITC in the watercress juice will lead to the depletion
of mutant p53 within the oral cells. This depletion could lead to a possible clinical
application of this compound, such as chemopreventives or oncologic treatments of
individuals with oral cancers.
Inclusion Criteria:
- Heavy Smoker
- Between the ages of 20 and 65
- Must be able to show proof of citizenship or residency of the United States
- Able to sign and understand a standard consent form
Exclusion Criteria:
- Pregnant or Breast-feeding
- Having any of the following medical conditions:
- Leukoplakia
- Oral Lesions
- Cancer
- Any oral disease that causes sores, ulcerations, irritations, etc.
- Stomach or Intestinal Ulcers
- IBS (Irritable Bowel Syndrome)
- Kidney Disease
- Allergies to watercress or other Cruciferous Vegetables
- Strict Vegetarians or Vegans
- Religious Consumers of Watercress or other Cruciferous Vegetable Juices
- On any of the following medications:
- Chlorzoxazone (Parafon Forte, Paraflex)
- Lithium
- Water Pills/Diuretics (Thiazide or Lasix)
- Warfarin (Coumadin)
- Vitamin K supplements
- H2 acid blocker (Zantac, Pepcid, Axid, Tagamet)
- Proton Pump Inhibitor (Prevacid)
- Digoxin
- Antibiotics
- Diabetic Medications (Insulin)
- Inhaled Bronchodilator
- Oral Steroids (Prednisone, Medrol)
- Inhaled Steroids (Flonase)
- Raloxifene or Tamoxifen
- Thyroid Hormone Replacement
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