Incretin Regulation of Insulin Secretion in Monogenic Diabetes

Monogenic diabetes patients and healthy matched controls will be admitted to the University
of Chicago Clinical Resource Center for a total of 4 nights. The following will take place
on separate days:

1. Oral Glucose Tolerance Test (OGTT): consume sugary drink and blood samples will be
collected at multiple time points

2. Isoglycemic glucose infusion (IGI) test: glucose will be infused via a vein in the arm
and and blood samples will be collected at multiple time points

3. OGTT during GLP-1 infusion

4. IGI during Exendin-9 infusion

Monogenic Diabetes Subjects:

Inclusion Criteria:

- Diagnosis of monogenic diabetes

- Previously participated in US Neonatal Diabetes Registry (IRB 15617B) or Genetics of
diabetes mellitus (IRB 6858)

- Age: 18 years +

Exclusion Criteria:

- Pregnancy

- Acute medical illness or chronic conditions including: cardiac failure, renal
insufficiency (estimated glomerular filtration rate <50 ml/min), hepatic
insufficiency (known cirrhosis or hepatitis), chronic obstructive pulmonary disease,
gastrointestinal disorders causing malabsorption, anemia (Hct < 36%), or uncontrolled
hypertension

Healthy Controls:

Inclusion Criteria:

- Good general health

- Stable weight for 6 months

- Age: 18 years +

Exclusion Criteria:

- Pregnancy

- Acute medical illness or chronic conditions including: cardiac failure, renal
insufficiency (estimated glomerular filtration rate <50 ml/min), hepatic
insufficiency (known cirrhosis or hepatitis), chronic obstructive pulmonary disease,
gastrointestinal disorders causing malabsorption, anemia (Hct < 36%), or uncontrolled
hypertension