Mechanisms of Response to Diesel Exhaust in Subjects With Asthma

The investigators will also characterize selected polymorphisms in glutathione-S-transferase
(GST) genes that have been implicated in susceptibility to air pollutants. Outcome measures
will include lung function, bronchial responsiveness to bronchodilator, exhaled nitric
oxide, induced sputum for inflammatory markers (IL6 and IL8), and exhaled breath condensate
for nitrite, 8-isoprostane, 20-HETE (20-hydroxyeicosatetraenoic acid), nitrotyrosine and pH.

In this pilot project, parallel studies are being carried out in the human exposure chambers
at two NIEHS centers, the EOHSI-CEED (Environmental and Occupational Health Sciences
Institute- Center for Environmental Exposure and Disease) and the Penn-CEET (University of
Pennsylvania-Center of Excellence in Environmental Toxicology). The joint project will
enable each center to validate research results in a second population and include new
biomarkers of pulmonary inflammation. Each center will recruit, consent, and collect data on
its own subjects. Only de-identified data will be shared between the centers.

Inclusion Criteria:

1. Subject is an adult male or female between 18 and 55 years of age inclusive.

2. Subject must have a prior history of a physician's diagnosis of asthma with
reversible airway obstruction (shown by a greater than 12% increase in FEV1 following
bronchodilator; or a positive methacholine challenge done following the screening
visit. If methacholine challenge is required for qualification, a physician will be
on-site for the test. Subject's asthma has been stable for the past six months with
no change in asthma therapy.

3. The subject has been a non-smoker for the past year and should have less than a
10-pack year history.

4. Subject must be free of other significant pulmonary diseases (i.e., cystic fibrosis,
tuberculosis, interstitial lung disease and bronchopulmonary dysplasia.

5. The subject must be capable of and willing to provide written informed consent.

6. The subject is able to understand and comply with protocol requirements and
timetables, instructions and protocol-stated restrictions.

7. The subject has no significant DE exposure that would include occupations such as
truck driver, heavy equipment operator and railroad maintenance.

Exclusion Criteria:

1. As a result of the medical interview, physical examination or screening
investigations, the physician responsible considers the volunteer unfit for the
study.

2. The subject has received an investigational drug or participated in any other
research trial within 30 days or five half-lives or twice the duration of the
biological effect of any drug (whichever is longer) prior to the current study.

3. Oral steroids within the last 6 months.

4. Less than 12% FEV1 response to bronchodilator or negative methacholine challenge.

5. Hospital admission for asthma in past 6 months.

6. The subject has a history of alcohol or drug abuse within the last 5 years.

7. The subject has history of hepatitis B, hepatitis C or HIV virus.

8. The subject has a history of cardiovascular disease including hypertension or says
yes to one of the cardiovascular risk questions on the screening questionnaire.

9. The subject has a history of diabetes.

10. The subject is pregnant or lactating.

11. The subject has daily exposure to DE, i.e., trucking or heavy machine operators.