A Proposed Study of Atherosclerotic Plaques in Leg Arteries
| Status: | Completed |
|---|---|
| Conditions: | Peripheral Vascular Disease, Cardiology |
| Therapuetic Areas: | Cardiology / Vascular Diseases |
| Healthy: | No |
| Age Range: | Any |
| Updated: | 7/1/2018 |
| Start Date: | June 2012 |
| End Date: | January 2017 |
Biofilm has been identified as the major bacterial phenotype contributing to atherosclerosis.
It has become very important to evaluate atherosclerosis and the role of biofilm using
advanced technologies. It is also important to understand wound biofilm at a genetic and a
molecular level.
It has become very important to evaluate atherosclerosis and the role of biofilm using
advanced technologies. It is also important to understand wound biofilm at a genetic and a
molecular level.
High throughput pyrosequencing is opening new doors in nucleic acid analysis. 454 Roche has
developed sequencing instrumentation which has the ability to sequence not only genomic DNA
(gDNA) but also derivatives of messenger RNA (mRNA) and 16s ribosomal DNA (rDNA). This
technology has the potential to survey the nucleic acids involved within a mixed population
of species, such as atherosclerotic biofilm. In addition to transcriptome science; proteome,
metabolome, and other -omic sciences are advancing rapidly. Examining atherosclerotic plaque
using transcriptome, proteome, metabolome, and other -omic approaches may provide valuable
insight into the wound's microecology.
developed sequencing instrumentation which has the ability to sequence not only genomic DNA
(gDNA) but also derivatives of messenger RNA (mRNA) and 16s ribosomal DNA (rDNA). This
technology has the potential to survey the nucleic acids involved within a mixed population
of species, such as atherosclerotic biofilm. In addition to transcriptome science; proteome,
metabolome, and other -omic sciences are advancing rapidly. Examining atherosclerotic plaque
using transcriptome, proteome, metabolome, and other -omic approaches may provide valuable
insight into the wound's microecology.
Inclusion Criteria:
1. The subject with identifying leg or legs that were amputated for vascular disease
and/or diabetes.
2. The subject must be 18 years of age or older.
Exclusion Criteria:
- The subject will be excluded if they have had a traumatic amputation which did not
involve vascular disease and/or diabetes.
We found this trial at
1
site
Click here to add this to my saved trials
