Evaluating Vitamin D Content in Mushrooms

Mushrooms have the capability to produce vitamin D in a similar way to human skin. When
exposed to ultraviolet light, mushrooms will convert a precursor to vitamin D. This reaction
produces large amounts of vitamin D. This study is comparing a natural source of vitamin D
to a synthetic source of vitamin D and will help determine if mushrooms are a novel source
for this essential nutrient.

Inclusion Criteria:

- All healthy adults, male and female, age 18-64 years

Exclusion Criteria:

- Currently taking, or having taken less than one month prior to start of study, a
prescription of 50,000 IU of vitamin D2 or 2000 IU vitamin D2 or vitamin D3

- Allergy to mushrooms

- History of elevated calcium (>10.4 mg%)

- Patients with a current or recent history of severe, progressive, and/or uncontrolled
renal, hepatic, hematological, endocrine, pulmonary, cardiac, neurological, or
cerebral disease.

- Supplementation with over the counter formulations of vitamin D2 or vitamin D3

- Subjects with a history of an adverse reaction to orally administered vitamin D.

- Subjects who are taking oral Dilantin or glucocorticoids.

- Exposure to a tanning bed or tanning on a beach for more than eight hours with no
sunscreen within 2 weeks prior to start of study.

- History of intestinal malabsorption (i.e. cystic fibrosis, fat malabsorption
syndrome, Crohn's Disease, gastric bypass surgery).

- Subjects with any other condition which in the Investigator's judgment would make the
patient unsuitable for inclusion in the study.