Evaluating the Expression Levels of MicroRNA-10b in Patients With Gliomas

MicroRNAs (miRNA) are molecular biomarkers that post-transcriptionally control target genes.
Deregulated miRNA expression has been observed in diverse cancers. In high grade gliomas,
known as glioblastomas, the investigators have identified an oncogenic miRNA, miRNA-10b
(mir-10b) that is expressed at higher levels in glioblastomas than in normal brain tissue.
This study tests the hypothesis that in primary glioma samples mir-10b expression patterns
will serve as a prognostic and diagnostic marker. This study will also characterize the
phenotypic and genotypic diversity of glioma subclasses. Furthermore, considering the
critical function of anti-mir-10b in blocking established glioblastoma growth, the
investigators will test in vitro the sensitivity of individual primary tumors to anti-mir-10b
treatment. Tumor, blood and cerebrospinal fluid samples will be obtained from patients
diagnosed with gliomas over a period of two years. These samples will be examined for mir-10b
expression levels. Patient survival, as well as tumor grade and genotypic variations will be
correlated to mir-10b expression levels.