Human MATER and Idiopathic Infertility
| Status: | Completed |
|---|---|
| Conditions: | Women's Studies, Infertility |
| Therapuetic Areas: | Reproductive |
| Healthy: | No |
| Age Range: | Any |
| Updated: | 4/21/2016 |
| Start Date: | May 2002 |
| End Date: | March 2011 |
Approximately 15 percent of couples experience infertility, yet no abnormalities can be
detected in the man or the woman. In a number of couples, their embryos unexpectedly slow
down growth or stop growth completely. Some of these situations may be genetically
determined. For instance, a portion of cases may be caused by poor egg quality related to
genetic or functional deficiencies in heretofore unidentified human maternal effect genes. A
model has been developed of such unexplained fertility by creating a mouse line lacking a
critical maternal effect gene. (Maternal effect genes produce mRNA or proteins that
accumulate in the egg and are required for normal early embryonic development.) This pilot
project will test the hypothesis that a similar defect may be a cause of human infertility.
Thirty cubic centimeters of blood will be collected from 40 women who have a clinical
history consistent with a defective maternal effect gene. DNA from these blood cells will be
examined and stored. Some of the blood cells will be treated so that they can be frozen and
grown in the laboratory to produce more DNA in the future. If certain mutations are not
found, that means that the prevalence of such mutations is less than 10 percent, and
investigators may initiate another study with 100 women. If a common mutation is found in at
least four patients, the investigators will seek to collect DNA from 150 normal fertile
control women for comparison.
This project is purely investigational; therefore, findings will not be shared with
participants.
detected in the man or the woman. In a number of couples, their embryos unexpectedly slow
down growth or stop growth completely. Some of these situations may be genetically
determined. For instance, a portion of cases may be caused by poor egg quality related to
genetic or functional deficiencies in heretofore unidentified human maternal effect genes. A
model has been developed of such unexplained fertility by creating a mouse line lacking a
critical maternal effect gene. (Maternal effect genes produce mRNA or proteins that
accumulate in the egg and are required for normal early embryonic development.) This pilot
project will test the hypothesis that a similar defect may be a cause of human infertility.
Thirty cubic centimeters of blood will be collected from 40 women who have a clinical
history consistent with a defective maternal effect gene. DNA from these blood cells will be
examined and stored. Some of the blood cells will be treated so that they can be frozen and
grown in the laboratory to produce more DNA in the future. If certain mutations are not
found, that means that the prevalence of such mutations is less than 10 percent, and
investigators may initiate another study with 100 women. If a common mutation is found in at
least four patients, the investigators will seek to collect DNA from 150 normal fertile
control women for comparison.
This project is purely investigational; therefore, findings will not be shared with
participants.
The pilot investigation will examine the hypothesis that human infertility may be caused by
mutations in the human MATER gene. We will determine the prevalence of these mutations in a
select group of women who have a clinical infertility history consistent with a possible
defect in a maternal effect gene. After obtaining informed consent and DNA from 100 women,
relevant mutations in the MATER gene will be searched for by single strand conformation
polymorphism analysis.
mutations in the human MATER gene. We will determine the prevalence of these mutations in a
select group of women who have a clinical infertility history consistent with a possible
defect in a maternal effect gene. After obtaining informed consent and DNA from 100 women,
relevant mutations in the MATER gene will be searched for by single strand conformation
polymorphism analysis.
- INCLUSION CRITERIA:
Women to be included in this study will have a clinical infertility history that would be
consistent with a possible defect in a maternal effect gene.
This includes women who meet the following criteria:
1. a clinical diagnosis of infertility,
2. never been pregnant, and
3. undergone treatment by in vitro fertilization and had at least 8 fertilized eggs as
part of at least one in vitro fertilization cycle that failed to lead to a clinical
pregnancy.
Women who have subsequently achieved a pregnancy by egg donation will be included.
Women of any age are eligible as long as they have otherwise met the inclusion criteria.
We found this trial at
1
site
9000 Rockville Pike
Bethesda, Maryland 20892
Bethesda, Maryland 20892
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