Detecting Infections Rapidly and Easily for Bacteremia Trial (DIREBT)

This protocol is intended for use by external sites collecting clinical research specimens
for T2 Biosystems' use in developing the T2Bacterial Assay. Specimens and information on
patients' medical history, blood properties, medications, and blood culture history/results
are required from the T2Bacterial Assay's target patient population (i.e., candidates for
blood culture). Ideally, clinical specimens for use in developing the T2Bacterial Assay will
be collected from negative blood culture, positive non-bacteria blood culture and positive
bacteria blood culture patients.

Inclusion Criteria:

- Subject or subject's authorized representative must be able to understand, read and
sign the study specific informed consent form after the nature of the study has been
fully explained to them.

- Males and females 18 - 95 years of age.

For Group A,

- Subject is confirmed to have bacteremia (gram negative rods, gram negative
coccobacillus, and/or gram positive cocci) as evidenced by a positive diagnostic
blood culture

- Subsequent species identification identifies the presence of one of the following 6
species of bacteria:

- Acinetobacter baumannii

- Staphylococcus aureus

- Klebsiella pneumonia

- Pseudomonas aeruginosa

- Enterococcus faecalis

- Enterococcus faecium

For Group B,

- Males and females 18 - 95 years of age.

- Subject is confirmed to not have bacteremia from the 6 bacteria species targeted by
this study, as evidenced by diagnostic blood culture and subsequent species
identification.

Exclusion Criteria:

- Use of any novel (i.e. not commercially available) drug compound within 30 days prior
to the collection of T2 blood specimens.

- Subject has other co-morbid condition(s) that, in the opinion of the Investigator,
could limit the subject's ability to participate in the study or impact the
scientific integrity of the study.

- Subject has had an anti-bacterial drug administered through the same port or central
line as is used to collect the clinical research specimens. A line draw cannot be
used to obtain a study sample if an antibacterial agent was administered
intravenously within the last 72 hours, unless the site can specifically document
that a different line was used to administer the anti-bacterial agent.