NECTAR Everolimus Plus Cisplatin in Triple (-) Breast Cancer

This is a phase II clinical trial of everolimus, an mTOR inhibitor, plus cisplatin
chemotherapy in patients with triple negative breast cancer (TNBC) who have residual disease
after completion of neoadjuvant chemotherapy. Everolimus and cisplatin will be administered
for 12 weeks. Patients will undergo surgery after treatment completion. Patients will have
breast biopsy prior to receiving the study treatment.

Inclusion Criteria:

1. Female patients ≥18 years of age.

2. Clinical/pathological documentation of residual disease after neo-adjuvant therapy.

3. Patients with synchronous bilateral cancers are eligible only if:

• Index cancer is triple-negative, defined as ER-, PR-, and HER2-.

4. HER2 negative tumors. HER2 negativity must be confirmed by one of the following:

- FISH-negative (FISH ratio <2.2), or

- IHC 0-1+, or

- IHC 2-3+ AND FISH-negative (FISH ratio <2.2).

5. Estrogen receptor negative and progesterone receptor negative (<10% staining by IHC
for estrogen receptor and progesterone receptor).

6. Eastern Cooperative Oncology Group (ECOG) Performance Status of 0 or 1.

7. Adequate hematologic function, defined by:

- Absolute neutrophil count 2 >1000/mm3

- Platelet count ≥100,000/mm3

- Hemoglobin >9 g/dL

8. Adequate liver function, defined by:

- AST and ALT ≤2.5 x the upper limit of normal (ULN)

- Total bilirubin ≤1.5 x ULN (unless the patient has grade 1 bilirubin elevation
due to Gilbert's disease or a similar syndrome involving slow conjugation of
bilirubin).

9. Adequate renal function, defined by:

• Serum creatinine ≤1.5 x ULN

10. Complete staging work-up ≤24 weeks prior to initiation of study treatment with
computed tomography (CT) scans of the chest and abdomen/pelvis (abdomen/pelvis
preferred; abdomen accepted), a CT scan of the head or MRI of the brain (if
symptomatic), and either a positron emission tomography (PET) scan or a bone scan.

11. Adequate cardiac function, defined by a left ventricular ejection fraction (LVEF)
value of >50% (or normal per institutional guidelines) by MUGA scan or echocardiogram
(ECHO).

12. Patients with previous history of invasive cancers (including breast cancer) are
eligible if definitive treatment was completed more than 5 years prior to initiating
current study treatment, and there is no evidence of recurrent disease.

13. Women of childbearing potential must have a negative serum or urine pregnancy test
performed within 7 days prior to start of treatment. If a woman becomes pregnant or
suspects she is pregnant while participating in this study, she must agree to inform
her treating physician immediately.

14. Patient must be accessible for treatment and follow-up.

15. Women of childbearing potential must agree to use an acceptable method of birth
control to avoid pregnancy for the duration of study treatment, and for 3 months
thereafter.

16. Able to swallow and retain oral medication.

17. Patient must be willing to undergo breast biopsies as required by the study protocol.

18. All patients must be able to understand the investigational nature of the study and
give written informed consent prior to study entry.

Exclusion Criteria:

1. Women who are pregnant or breastfeeding.

2. History of previously treated ductal carcinoma in situ (DCIS) is acceptable.

3. Malabsorption syndrome, disease significantly affecting gastrointestinal function, or
resection of the stomach or small bowel.

4. Known intolerance or hypersensitivity to Everolimus or other rapamycin analogs (e.g.
sirolimus, temsirolimus);

5. Previous cancer (with the exception of non-melanoma skin cancer or cervical carcinoma
in situ) in the past 5 years.

6. Patients who have any severe and/or uncontrolled medical conditions such as:

1. unstable angina pectoris, symptomatic congestive heart failure, myocardial
infarction ≤6 months prior to start of Everolimus, serious uncontrolled cardiac
arrhythmia, or any other clinically significant cardiac disease

2. Symptomatic congestive heart failure of New York heart Association Class III or
IV

3. active (acute or chronic) or uncontrolled severe infection, liver disease such as
cirrhosis, decompensated liver disease, and chronic hepatitis (i.e. quantifiable
HBV-DNA and/or positive HbsAg, quantifiable HCV-RNA),

4. known severely impaired lung function (spirometry and DLCO 50% or less of normal
and O2 saturation 88% or less at rest on room air),

5. active, bleeding diathesis;

7. Patients may not receive any other investigational or anti-cancer treatments while
participating in this study.

8. Concurrent severe, uncontrolled infection or intercurrent illness including, but not
limited to, ongoing or active infection, or psychiatric illness/social situations that
would limit compliance with study requirements.

9. Mental condition that would prevent patient comprehension of the nature of, and risk
associated with, the study.

10. Inability to comply with study and/or follow-up procedures.

11. Patients who have received live attenuated vaccines within 1 week of start of
Everolimus and during the study. Patient should also avoid close contact with others
who have received live attenuated vaccines.

Examples of live attenuated vaccines include intranasal influenza, measles, mumps,
rubella, oral polio, BCG, yellow fever, varicella and TY21a typhoid vaccines;

12. Known history of HIV seropositivity;

13. Women of child-bearing potential (WOCBP), defined as all women physiologically capable
of becoming pregnant, unless they are using highly effective methods of contraception
during dosing of study treatment. Highly effective contraception methods include
combination of any two of the following (a+b or a+c or b+c):

1. Use of oral, injected or implanted hormonal methods of contraception or;

2. Placement of an intrauterine device (IUD) or intrauterine system (IUS);

3. Barrier methods of contraception: condom or occlusive cap (diaphragm or
cervical/vault caps) with spermicidal foam/gel/film/cream/ vaginal suppository;

4. Total abstinence or;

5. Male/female sterilization. Women are considered post-menopausal and not of
child-bearing potential if they have had 12 months of natural (spontaneous)
amenorrhea with an appropriate clinical profile (e.g. age appropriate, history of
vasomotor symptoms) or have had surgical bilateral oophorectomy (with or without
hysterectomy) or tubal ligation at least six weeks prior to treatment. In the
case of oophorectomy alone, only when the reproductive status of the woman has
been confirmed by follow up hormone level assessment is she considered not of
child-bearing potential.

14. Chronic treatment with corticosteroids or other immunosuppressive agents. Topical or
inhaled corticosteroids are allowed;