Acceleration of Insulin Action by Hyaluronidase During Closed-Loop Therapy

To investigate the effect of rHuPH20, an adjuvant that accelerates the dispersion and
absorption of subcutaneously injected or infused drugs, on mitigating post-prandial blood
glucose excursions when injected separately or co-formulated with insulin during closed-loop
therapy for youth and young adults with type 1 diabetes. Closed-loop control will be
achieved using external subcutaneous real-time continuous glucose monitoring and continuous
subcutaneous insulin infusion along with a computerized algorithm to link these two
processes.

Specific Aim 1: To examine whether co-formulation rHuPH20 with analog insulin (INS-PH20)or,
alternately, pre-administration of rHuPH20 (PH20-preRx) at the time of infusion set
placement prior to initiation of closed-loop (CL) insulin delivery will reduce
peak-postprandial glucose concentrations and total glucose area under the curve of the meal
excursions in short term inpatient experiments.

Specific Aim 2: To investigate whether accelerated insulin absorption by rHuPH20, delivered
as described above, will also result in a reduction of late-post-prandial hyperinsulinemia
and late post-prandial hypoglycemia during CL insulin delivery.

Specific Aim 3: To compare the insulin accelerator effect of INS-PH20 to that of PH20-preRx,
based on post prandial glucose excursions during closed-loop therapy

We hypothesize that; utilization of PH20 either as a separate injection (PH20-preRx) or in a
co-formulation with insulin (INS-PH20) during CL therapy will reduce peak-postprandial
glucose concentrations and total glucose under the curve of the meal excursion as compared
to CL control without any intervention, and we propose that the use of PH20-preRx and
INS-PH20 will be well tolerated when delivered in youth and young adults in a closed-loop
setting.

Inclusion Criteria:

1. age 12-40 years

2. clinical diagnosis of T1D based on ADA criteria or presence of DKA at diagnosis
(formal antibody and/or genetic testing will not be required)

3. duration of T1D ≥ 1 year

4. HbA1c ≤ 9 %

5. Treated with CSII for at least 3 months

6. Body weight > 37 kg (to accommodate phlebotomy)

7. Normal hematocrit

8. Normal creatinine

9. Not pregnant or lactating, and for female subjects of reproductive potential, are
abstinent or are consistently using barrier or hormonal methods of contraception

Exclusion Criteria:

1. Insulin resistant (defined as requiring > 2 units/kg/day at time of study enrollment

2. Previous allergic reaction to PH20

3. Inability to comprehend written or spoken English

4. Presence of any medical or psychiatric disorder that may interfere with subject
safety or study conduct

5. Use of any medications (besides insulin) known to affect blood glucose levels,
including oral or other systemic glucocorticoid therapy. Inhaled, intranasal, or
rectal corticosteroid use is allowed along as not given within 4 weeks of admission
to the HRU. Use of topical glucocorticoids is allowable as long as affected skin area
does not overlap with study device sites. Subjects using herbal supplements will be
excluded, due to the unknown effects of these supplements on glucose control

6. Use of furosemide, benzodiazepines or phenytoin during the study

7. History of poor wound healing, heat sensitivity, or diminished skin integrity.

8. History of hypoglycemic seizure within last 3 months

9. Anemic (low hematocrit), or evidence of renal insufficiency (elevated serum
creatinine)

10. Female subjects who are pregnant, lactating, or unwilling to be tested for pregnancy

11. Subjects unable to give consent / permission / assent