Validation of the Short-term Antimicrobial Action of Transplanted Bacteria
| Status: | Withdrawn |
|---|---|
| Conditions: | Psoriasis, Dermatology, Dermatology |
| Therapuetic Areas: | Dermatology / Plastic Surgery |
| Healthy: | No |
| Age Range: | 18 - 80 |
| Updated: | 8/24/2018 |
| Start Date: | January 2015 |
| End Date: | July 2017 |
Phase 1 Study of the Short-term Antimicrobial Action of Transplanted Bacteria in Adult Patients With Atopic Dermatitis
Unlike healthy control skin, the skin of patients with atopic dermatitis (AD) is frequently
colonized by Staphylococcus aureus (S. aureus), putting these patients at increased risk of
S. aureus skin infections. In addition, research in the investigator's lab has shown that
these patients have fewer protective antimicrobial Staphylococcal species such as
Staphylococcal epidermidis (S. epidermidis) that are known to produce antimicrobial peptides
that play a role in protecting the skin from invading pathogens. In this study, the
investigator will attempt to decrease S. aureus colonization and increase colonization by
protective Staph species in AD patients. First the investigator will capture the bacteria on
subjects' lesional AD skin. Next the investigator will selectively grow the subject's
antimicrobial Staphylococcal colonies and place them into a base moisturizer. The moisturizer
plus bacteria will be applied to one of the subject's arms, and the moisturizer alone
(without bacteria) to the other arm. The investigator will then do a quantitative wash of the
bacteria growing on each arm one day later in order to determine whether the S. aureus
abundance was affected by the application of the transplanted bacteria.
colonized by Staphylococcus aureus (S. aureus), putting these patients at increased risk of
S. aureus skin infections. In addition, research in the investigator's lab has shown that
these patients have fewer protective antimicrobial Staphylococcal species such as
Staphylococcal epidermidis (S. epidermidis) that are known to produce antimicrobial peptides
that play a role in protecting the skin from invading pathogens. In this study, the
investigator will attempt to decrease S. aureus colonization and increase colonization by
protective Staph species in AD patients. First the investigator will capture the bacteria on
subjects' lesional AD skin. Next the investigator will selectively grow the subject's
antimicrobial Staphylococcal colonies and place them into a base moisturizer. The moisturizer
plus bacteria will be applied to one of the subject's arms, and the moisturizer alone
(without bacteria) to the other arm. The investigator will then do a quantitative wash of the
bacteria growing on each arm one day later in order to determine whether the S. aureus
abundance was affected by the application of the transplanted bacteria.
Inclusion Criteria:
- Male or female subjects who are not pregnant or lactating
- 18-80 years of age
- Diagnosis of atopic dermatitis for at least 6 months using the Hanifin and Rajka
Diagnostic Criteria for atopic dermatitis
- Presence of lesional atopic dermatitis skin in both antecubital fossae
- Positive methicillin-sensitive S. aureus colonization based on results of a skin
culture taken from one of their AD-affected antecubital fossae during the screening
visit
Exclusion Criteria:
- Use of any topical AD treatments (including topical steroids, topical calcineurin
inhibitors) to either arm within one week of either screening visit
- Use of any oral/systemic AD therapies (antihistamines, steroids) within 28 days of
either screening visit
- Severe AD that would worsen significantly from holding a participant's usual
topical/oral AD medications for the time periods required in the inclusion/exclusion
criteria (one week prior to the screening visits and during the study for topical
medications and 28 days prior to screening visits and during the study for oral
medications)
- Subjects who have taken a bleach bath within a week prior to screening, or who take
bleach baths during the study
- Patients with severe medical condition(s) that in the view of the investigator
prohibits participation in the study
- Subjects with Netherton's syndrome or other genodermatoses that result in a defective
epidermal barrier
- Any subject who is immunocompromised (e.g. provides researchers with a history
lymphoma, HIV/AIDS, Wiskott-Aldrich Syndrome) or has a history of malignant disease
(with the exception of non-melanomatous skin cancer). This information will be
gathered verbally from the patient while taking a medical history from the patient,
and will not involve further testing such as an HIV test.
- Subjects with a history of psychiatric disease or history of alcohol or drug abuse
that would interfere with the ability to comply with the study protocol
- Active bacterial, viral or fungal skin infections
- Any noticeable breaks or cracks in the skin on either arm, including severely
excoriated skin or skin with open or weeping wounds suggestive of an active infection
or increased susceptibility to infection.
- Ongoing participation in another investigational trial
- Use of any oral or topical antibiotic for up to four weeks prior to screening
- Use of any systemic immunosuppressive therapy (e.g. cyclosporin, methotrexate, etc.)
within four weeks of screening.
- Sensitivity to or difficulty tolerating Dove fragrance-free bar soap, Dial
antibacterial liquid soap, Cetaphil lotion, or alcohol-based cleaners
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