Exercise-Induced Epigenetic Modifications in Obese Aging Women
| Status: | Completed |
|---|---|
| Conditions: | Endocrine |
| Therapuetic Areas: | Endocrinology |
| Healthy: | No |
| Age Range: | 50 - 64 |
| Updated: | 4/21/2016 |
| Start Date: | June 2013 |
| End Date: | December 2014 |
Our greatest public health challenge is obesity and the co-morbidities of metabolic syndrome
(MetS). Age is an established risk factor for MetS and specific to women, data indicates
that the prevalence of MetS increases substantially with the menopausal transition with
postmenopausal women having a 60% increased risk of MetS. Menopause also contributes to
reductions in strength, physical function and often psychological well-being (e.g. fatigue).
Obese individuals also have: a) impaired immune function and chronic inflammatory responses
associated with changes in the white blood cell population in blood and fat tissues; and, b)
increased secretion of and signaling by proteins in their fat cells. Weight loss, which
requires an energy deficit through increased physical activity and/or caloric restriction
(EX+CR), reduces risk for MetS in older sedentary obese women by reducing insulin resistance
and chronic systemic inflammation. Science and clinical practice will be advanced by
examining the molecular mechanisms by which EX+CR affects risk for MetS in older women. The
primary aim is to determine if CD4+ T cells will report the differential epigenetic
reprogramming of relevant gene expression associated with metabolic indices resulting from
EX+CR induced weight loss in older women known to be at risk for MetS. This pilot data will
be used to generate an NIH proposal of the same topic. A secondary aim is to assess the
impact of weight loss on physical function and psychological well-being which will provide
pilot data for an additional grant proposal regarding weight management in postmenopausal
women.
(MetS). Age is an established risk factor for MetS and specific to women, data indicates
that the prevalence of MetS increases substantially with the menopausal transition with
postmenopausal women having a 60% increased risk of MetS. Menopause also contributes to
reductions in strength, physical function and often psychological well-being (e.g. fatigue).
Obese individuals also have: a) impaired immune function and chronic inflammatory responses
associated with changes in the white blood cell population in blood and fat tissues; and, b)
increased secretion of and signaling by proteins in their fat cells. Weight loss, which
requires an energy deficit through increased physical activity and/or caloric restriction
(EX+CR), reduces risk for MetS in older sedentary obese women by reducing insulin resistance
and chronic systemic inflammation. Science and clinical practice will be advanced by
examining the molecular mechanisms by which EX+CR affects risk for MetS in older women. The
primary aim is to determine if CD4+ T cells will report the differential epigenetic
reprogramming of relevant gene expression associated with metabolic indices resulting from
EX+CR induced weight loss in older women known to be at risk for MetS. This pilot data will
be used to generate an NIH proposal of the same topic. A secondary aim is to assess the
impact of weight loss on physical function and psychological well-being which will provide
pilot data for an additional grant proposal regarding weight management in postmenopausal
women.
Inclusion Criteria:
1. Volunteer for the study
2. Age between 50 and 64 years
3. Self-identified as White or Caucasian
4. Postmenopausal
5. Sedentary (less than 1 hour each week of planned physical activity and sedentary job)
6. BMI range >/= 25 kg/m2
7. Waist circumference >88cm
8. Weight stable (within 2 kg) for 6 months
9. All allowable medications stable for 3 months
10. Live independently
11. Willing and able to obtain transportation to and from lab sessions
12. Obtains physician clearance to participate in the study
Exclusion Criteria:
1. Tobacco use
2. Normal weight (BMI < 25 kg/m2)
3. Males
4. Dietary restrictions that do not allow for the consumption of beef, as required by
our dietary protocol
5. Weight loss surgery and/or weight loss medications usage
6. Mini-mental state exam score < 25
7. Recent or history of unstable CVD
8. Cancer treatment within the last 5 years or active cancer
9. History of lung disease or COPD or severe asthma
10. History or severe arthritis or other medical condition that precludes ability to
exercise to level needed by study.
11. Current diagnosis or history of balance disorders
12. History of mental disorders, dementia, clinical depression or other disorders that
preclude adherence to protocols.
13. Current weight of 350 pounds or greater, due to weight restrictions on equipment.
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