Autologous Cultured Myoblasts (BioWhittaker) Transplanted Via Myocardial Injection

MyoCell™ mediated cellular cardiomyoplasty is a novel therapeutic approach to the management
of progressive heart failure in patients who have damaged myocardial tissue resulting from a
myocardial infarct. MyoCell™ consists of patient autologous skeletal myoblasts which are
expanded ex vivo and supplied as a cell suspension in a buffered salts solution for
injection into the area of damaged, akinetic myocardium with the goal of having the
myoblasts populate the implant area and generate elastic, contractile skeletal muscle-like
tissue within the damaged myocardium. Because the physiological goal is to replace
inelastic, fibrous myocardial scar tissue with skeletal muscle-like tissue, originating from
the cellular implants, this therapeutic approach is termed "cellular cardiomyoplasty" or
"CCM".

The purpose of this trial is to assess the safety of MyoCell™(expanded autologous skeletal
myoblasts) using a dose escalation methodology following epicardial injection into
myocardial scar tissue in patients who have experienced anterior, lateral, posterior or
inferior wall myocardial infarction, require coronary artery bypass graft (CABG) surgery and
who have an implantable cardioverter defibrillator (ICD) in place (ICD can be implanted
during the CABG procedure or 3 to 4 days post CABG procedure). Safety endpoints will be the
evaluation of the nature and frequency of Adverse Events during the 12-month period
following MyoCell™ treatment.

If a patient meets the baseline enrollment criteria, a 5-10 gram skeletal muscle biopsy will
be obtained for myoblast isolation and expansion in vitro at Bioheart's designated facility
for MyoCell™ production. Biopsy will occur 3 - 4 weeks prior to the anticipated implantation
of the MyoCell™ product. At the time of the patient's CABG surgery MyoCell™ will be injected
into the akinetic myocardial scar in the region of a previous infarct utilizing a sterile
hypodermic syringe fitted with a 25 gauge needle.

This will be a dose escalation study with 3 cohort groups consisting of 5 patients each. A
report of the 1 month safety data from each cohort will be presented to the data safety
monitoring board for permission to go to the next higher dosage. In the first cohort of this
dose escalation study; 2 injections will be performed, for the second cohort; 6 injections
and for the third cohort; 18 injections depending on the size of the infarct scar, so as to
inject the entire myocardial infarct scar akinetic area.

Inclusion Criteria:

- Defined region of myocardial dysfunction related to previous myocardial infarction
involving the anterior, lateral, posterior or inferior walls, > 12 weeks old at the
scheduled time of MyoCell™ implantation procedure

- Patients must have an implantable cardioverter defibrillator (ICD) in place or,
patients must receive ICD placement during the CABG procedure or 3 to 4 days after
the CABG procedure

- Planned CABG procedure for revascularization

- Heart failure patient in New York Heart Association Symptom Class 2 or 3 who is on
optimal medical therapy

- Age > 18 and < 80 years

- Able to undergo surgical biopsy of the skeletal muscle and successful culture of the
harvested myoblasts

- Target region wall thickness > 6 mm by echocardiography

- Left ventricular ejection fraction > 20% and < 40% by radionuclide ventriculography
or left ventricular angiography at screening

- If a female of childbearing potential, urine pregnancy test must be negative

- Able to give written informed consent

Exclusion Criteria:

- Patient medically unable to undergo CABG surgery

- Any patient who has had a documented myocardial infarction (ECG changes or elevated
cardiac enzymes consistent with MI) within 30 days of the scheduled surgical
revascularization and cellular implantation procedure.

- Known sensitivity to gentamicin sulfate and/or amphotericin-B

- Exposure to any investigational drug or procedure within 4 weeks prior to study entry

- The use or expected use of antineoplastic drugs

- History of skeletal muscle disease, either primary (i.e., myopathy) or secondary
(i.e., ischemic) or any underlying myopathy such as myasthenia gravis, muscular
dystrophy, etc.) as determined by a board certified pathologist examining sample of
patients muscle biopsy

- Previous angiogenic therapy and/or myocardial laser therapy

- History of cancer within 5 years, except for basal cell carcinoma of the skin

- PSA suggestive of carcinoma of the prostate (i.e., > 4)

- Patient with CEA >2.5 ng/mL or end stage renal disease

- Patients with active infectious disease and/or who are known to have tested positive
for HIV, HTLV, HBV-sAg, HCV, CMV and/or syphilis. If the panel includes antibodies to
the HBc and HBV-sAg, then an expert will be consulted.

- Females who are pregnant or nursing. Females of childbearing potential must be using
to the investigator's satisfaction, a medically accepted method of birth control
(e.g., but not limited to, oral or implanted contraceptive therapy or intrauterine
devices) and agree to continue for the duration of the study.

- Any illness which might affect patient’s survival over the study follow-up period

- Any illness which, in the Investigator’s judgment, will interfere with the patient’s
ability to comply with the protocol, compromise patient safety, or interfere with the
interpretation of the study results.

- Patient with an any previous cardiac surgery

- Patient with valvular disease greater than mild in degree which is considered the
primary cause of their heart failure

- Patient in New York Heart Association Symptom Class 1 or 4

- Patient with serum creatinine > 2.5

- No informed consent or unable to provide informed consent