RNAseq Analysis of Microbial Gene Expression in Necrotizing Soft Tissue Infections
| Status: | Recruiting |
|---|---|
| Conditions: | Skin and Soft Tissue Infections, Infectious Disease |
| Therapuetic Areas: | Dermatology / Plastic Surgery, Immunology / Infectious Diseases |
| Healthy: | No |
| Age Range: | 18 - Any |
| Updated: | 1/10/2019 |
| Start Date: | December 2013 |
| End Date: | December 2019 |
| Contact: | Kendra Rumbaugh, PhD |
| Email: | kendra.rumbaugh@ttuhsc.edu |
| Phone: | 806-743-2460 |
Hypothesis: We hypothesize that RNAseq will allow us to learn more about the causative agents
of necrotizing soft tissue infections (NSTI) and risk factors involved in acquiring these
devastating infections
Tissue biopsies will be obtained from consented patients admitted with NSTI. Biopsies will be
used for standard clinical analysis of bacterial species present or stored for later RNA
sequencing. RNA sequencing will identify bacteria that are present within the infection site
that may not be detected using standard culture techniques as well as reveal bacterial gene
expression profiles within the NSTI site. Medical charts will also be reviewed for basic
patient information as well as wound care management practices. We hope to identify bacterial
species commonly present in these types of infections as well as risk factors predisposing
individuals to NSTI's.
of necrotizing soft tissue infections (NSTI) and risk factors involved in acquiring these
devastating infections
Tissue biopsies will be obtained from consented patients admitted with NSTI. Biopsies will be
used for standard clinical analysis of bacterial species present or stored for later RNA
sequencing. RNA sequencing will identify bacteria that are present within the infection site
that may not be detected using standard culture techniques as well as reveal bacterial gene
expression profiles within the NSTI site. Medical charts will also be reviewed for basic
patient information as well as wound care management practices. We hope to identify bacterial
species commonly present in these types of infections as well as risk factors predisposing
individuals to NSTI's.
Inclusion Criteria:
- Patient or LAR agrees to the procedure and signs informed consent form
- Patient is at least 18 yrs of age
- Patient has been diagnosed with an NSTI
Exclusion Criteria:
- Patient not willing to undergo additional tissue biopsies for RNA sequencing analysis
We found this trial at
2
sites
Lubbock, Texas 79430
Principal Investigator: Kendra Rumbaugh, PhD
Phone: 806-743-2460
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