Intensive Insulin Therapy With Tight Glycemic Control to Improve Outcomes After Endovascular Therapy for Acute Ischemic Stroke

This will be a prospective open label study of 100 subjects who will be randomized to either
an insulin aspart (study drug) infusion (intensive insulin therapy [IIT arm]) for 48hrs or
to a control arm. All patients attending the emergency department (ED) of Kaleida health
within 24 hrs of symptom onset suggestive of an anterior circulation ischemic stroke will be
screened. If they meet the inclusion criteria and do not have any exclusion criteria,
informed consent will be obtained and a blinded stratified block randomization process will
be initiated.

In addition to receiving endovascular and standard medical therapy for AIS, subjects will be
randomized to an intensive insulin treatment arm (IIT arm) or control arm. In the IIT arm
subjects will receive an insulin aspart infusion at a minimal rate of 2 units/hr while
maintaining blood glucose between 90-120mg/dl for 48 hrs. Glucose control in the control
will be at the discretion of the treating provider.

Subjects will have the following study procedures done at different time points: A)
assessment of neurological outcomes : modified Rankin scale (mRS) at 30 days and 90 days and
NIH stroke scale (NIHSS) at baseline , 24 hrs, 72 hrs, 30 days and 90 days.

B) MRI (Diffusion weighted imaging), 3-5 days and 90 days and C) various plasma and cellular
endpoints will be measured in blood collected at baseline, 2, 4, 6, 24 , 48 and 72 hrs and
at 30 days and 90 days.

The primary endpoint of the study is to detect a difference in the percentage of non
diabetic subjects with mRS 0-2 at 90 days after an AIS treated with endovascular therapy, in
the IIT and control arm.

Inclusion Criteria:

1. Age 18-85 years

2. All patients attending the emergency department (ED) of Kaleida health within 24 hrs
of symptoms onset suggestive of an anterior circulation ischemic stroke.

3. CT perfusion suggesting Ischemic Core less than 30% of Penumbra territory.

4. No history of diabetes

5. First neurological event

6. Clinical signs consistent with the diagnosis of ischemic stroke, including impairment
of language, motor function, cognition and/or gaze, vision, or neglect. Ischemic
stroke is defined as an event characterized by the sudden onset of a focal neurologic
deficit presumed to be due to cerebral ischemia after exclusion of ICH with a
baseline CT

7. The signal stroke should be (a) acute, (b) the most recent significant, acute
worsening of serial neurologic events, or (c) related to a diagnostic radiographic
procedure but not an interventional procedure

8. Minimum NIHSS score >4, except for isolated aphasia or isolated hemianopsia

9. Angiographic evidence of a clot in the anterior intracranial or extracranial
circulation consistent with the neurologic deficit with complete occlusion (TICI
grade 0) or contrast penetration with minimal perfusion (TICI grade 1).

10. Signed informed consent to participate given by patient or legal representative.

Exclusion Criteria:

1. Coma

2. Neurologic signs that are rapidly improving by the time of randomization or
treatment- a 4-point improvement from baseline NIHSS , or increase to absolute NIHSS
> 30 before randomization or treatment

3. Major stroke symptoms- NIHSS >30

4. Seizure at the onset of stroke

5. Stroke due to a neurointerventional procedure for treatment of a cerebral aneurysm
and/or cerebral arteriovenous malformation (stroke due to diagnostic cerebral
angiography or cardiac catheterization might be treated)

6. Clinical presentation suggestive of subarachnoid hemorrhage, even when the initial CT
scan is normal.

7. Previous known ICH at any time, neoplasm, and/or subarachnoid hemorrhage.

8. Patients with a known arteriovenous malformation or aneurysm, with or without any
evidence of associated hemorrhage.

9. Presumed septic embolus

10. Known hereditary or acquired hemorrhagic diathesis, eg, aPTT or prothrombin time
greater than normal; unsupported coagulation factor deficiency.

11. Baseline laboratory values that reveal platelets are <30 000/µL, hematocrit or
platelet cell volume <25 volume %, or international normalized ratio >1.7. (Any
patient receiving heparin at the onset of stroke symptoms must have an aPTT 2 times
the upper limit of normal before randomization. Patients receiving
low-molecular-weight heparin might need to be excluded because an anticoagulant
effect is not measured by aPTT.)

12. Pregnancy, lactation, or parturition within the previous 30 days.

13. Known serious sensitivity to radiographic contrast agents.

14. Other serious, advanced, or terminal illness such that life expectancy is <1 year.

15. Current participation in another research treatment protocol.

16. Previous participation in an acute stroke study.

17. Any condition in which angiography is contraindicated.

18. Uncompensated hypertension at study entry or hypertension requiring aggressive
treatment to reduce blood pressure to non-hypertensive limits. Uncompensated
hypertension is defined as systolic blood pressure >180 mm Hg or diastolic blood
pressure 100 mm Hg on 3 repeated measures at least 10 minutes apart. Aggressive
treatment is defined as the need for a continuous, parenteral antihypertensive, such
as a nitroprusside drip, or the need to administer >3 doses of a parenteral
antihypertensive, such as labetalol, hydralazine, nicardepine.

19. Dependency on renal dialysis or known serum creatinine > 2.0mg/dl

20. Serum glucose at admission <80mg/dl

21. All known diabetic patients

22. Random Admission glucose > 200mg/dl

23. High-attenuation lesion on CT consistent with a hemorrhage of any degree in any
location.

24. Evidence of a significant mass effect with a midline shift due to a large infarct

25. Acute hypodense parenchymal lesion on CT or effacement of the cerebral sulci in more
than one third of the MCA territory or suspected stroke region

26. Angiographic evidence of (a) Suspected carotid arterial dissection. (b) Arterial
stenosis as the sole lesion or a high-grade stenosis that does not allow safe passage
of a catheter. (b) Any nonatherosclerotic arteriopathy (eg, vasculitis)

27. Mentally incompetent and wards of the state.