Smoking, Stress & Allopregnanolone Response

Cigarette smoking persists as the leading cause of preventable morbidity and mortality.
Thus, finding ways to maximize quitting behavior is critical. Women have a more difficult
time achieving smoking abstinence than men. The risk for smoking relapse appears to vary by
menstrual phase such that the follicular phase is associated with greater risk compared to
the luteal phase. Women are also more likely to smoke in response to stressful stimuli than
men. Allopregnanolone (ALLO) is a stress-reducing neuroactive steroid that is primarily
metabolized from the sex hormone progesterone and, therefore, varies by menstrual phase in
women. Recent preclinical literature has indicated that ALLO may protect against drug abuse
behaviors. Unfortunately, ALLO remains largely unexplored in clinical samples containing
women.

In this study we aim to characterize the effect of smoking on stress-induced ALLO response
in women by menstrual phase. To achieve this goal, we will recruit a sample of premenopausal
women who smoke (n=30) and do not smoke (n=30) to participate in a controlled cross-over
study. All participants will complete two four-hour lab sessions timed to occur in the
Follicular (F; low ALLO) and Luteal (L; high ALLO) menstrual phases. Each lab session will
contain an acute stressor along with a timed series of assessments including blood samples
(for ALLO measurement) and self-report of mood and perceived stress.

Inclusion Criteria:

- Female between the ages of 18-40 years old

- Stable physical/mental health,

- Self-report of regular menstrual cycles,

- Self-report of either smoking either current or never smoking,

- English fluency,

- Ability to provide informed consent

Exclusion Criteria:

- Self-report of current use of illicit drugs, other tobacco products, nicotine, or
smoking cessation medications,

- Current or recent pregnancy or breastfeeding,

- Current or recent use of exogenous hormones (including birth control pills),

- Current or recent use of psychotropic medications.