A Multicenter Study of Outpatient Automated Blood Glucose Control With a Bihormonal Bionic Pancreas

Inclusion Criteria:

- Age ≥18 years and have had clinical type 1 diabetes for at least one year

- Diabetes managed using an insulin pump for ≥ 6 months

- Prescription medication regimen stable for > 1 month (except for medications that will
not affect the safety of the study and are not expected to affect any outcome of the
study, in the judgement of the site principal investigator).

- Employee or student working or studying during most of the week at one of the
participating campuses (Massachusetts General Hospital in Boston, MA; University of
Massachusetts Medical Center in Worcester, MA; University of North Carolina in Chapel
Hill, NC; Stanford University in Palo Alto, CA)

- Lives within a 30 minute drive-time radius of the central monitoring location for one
of the study sites

- Willing to remain within a 60 minute drive-time radius of the central monitoring
location for one of the study sites during each of the 11-day study arms

- Have someone over 18 years of age who lives with them, has access to where they sleep,
is willing to be in the house when the subject is sleeping, and is willing to receive
calls from the study staff and check the welfare of the study subject if telemetry
shows a technical problem or severe biochemical hypoglycemia without subject response
and the subject does not answer their telephone (up to two individuals can share this
role, but they must be willing to carefully coordinate with each other and the subject
so that one of them is clearly designated as having this responsibility at any given
time)

- Willing to wear two infusion sets and continuous glucose monitor (CGM) sensor and
change sets frequently (at least one new glucagon infusion set daily)

Exclusion Criteria:

- Unable to provide informed consent (e.g. impaired cognition or judgment)

- Unable to safely comply with study procedures and reporting requirements (e.g.
impairment of vision or dexterity that prevents safe operation of the bionic pancreas,
impaired memory, unable to speak and read English)

- Current participation in another diabetes-related clinical trial that, in the judgment
of the principal investigator, will compromise the results of this study or the safety
of the subject

- Pregnancy [positive urine human chorionic gonadotropin (HCG)] breast feeding, plan to
become pregnant in the immediate future, or sexually active without use of
contraception

- Need to go outside of the designated geographic boundaries during either arm of the
study

- Current alcohol abuse (intake averaging > 3 drinks daily in last 30 days), use of
marijuana within 1 month of enrollment, or other substance abuse (use within the last
6 months of controlled substances other than marijuana without a prescription)

- Unwilling or unable to refrain from drinking more than 2 drinks in an hour or more
than 4 drinks in a day or use of marijuana during the trial

- Unwilling or unable or to avoid use of drugs that may dull the sensorium, reduce
sensitivity to symptoms of hypoglycemia, or hinder decision making during the period
of participation in the study (use of beta blockers will be allowed as long as the
dose is stable and the subject does not meet the criteria for hypoglycemia unawareness
while taking that stable dose, but use of benzodiazepines or narcotics, even if by
prescription, may be excluded according to the judgment of the principal investigator)

- History of liver disease that is expected to interfere with the anti-hypoglycemia
action of glucagon (e.g. liver failure or cirrhosis). Other liver disease (i.e. active
hepatitis, steatosis, active biliary disease, any tumor of the liver, hemochromatosis,
glycogen storage disease) may exclude the subject if it causes significant compromise
to liver function or may do so in an unpredictable fashion.

- Renal failure on dialysis

- Personal history of cystic fibrosis, pancreatitis, pancreatic tumor, or any other
pancreatic disease besides type 1 diabetes

- Any known history of coronary artery disease including, but not limited to, history of
myocardial infarction, stress test showing ischemia, history of angina, or history of
intervention such as coronary artery bypass grafting, percutaneous coronary
intervention, or enzymatic lysis of a presumed coronary occlusion)

- Abnormal electrocardiogram (EKG) consistent with coronary artery disease or increased
risk of malignant arrhythmia including, but not limited to, evidence of active
ischemia, prior myocardial infarction, proximal left anterior descending coronary
artery (LAD) critical stenosis (Wellen's sign), prolonged QT interval (> 440 ms).
Non-specific ST segment and T wave changes are not grounds for exclusion in the
absence of symptoms or history of heart disease. A reassuring evaluation by a
cardiologist after an abnormal EKG finding may allow participation.

- Congestive heart failure (CHF) [established history of CHF, lower extremity edema,
paroxysmal nocturnal dyspnea, or orthopnea]

- History of transient ischaemic attack (TIA) or stroke

- Seizure disorder, history of any non-hypoglycemic seizure within the last two years,
or ongoing treatment with anticonvulsants

- History of hypoglycemic seizures or coma in the last year

- History of pheochromocytoma: fractionated metanephrines will be tested in patients
with history increasing the risk for a catecholamine secreting tumor:

- episodic or treatment refractory (requiring 4 or more medications to achieve
normotension) hypertension

- paroxysms of tachycardia, pallor, or headache

- personal or family history of multiple endocrine neoplasia type 2A (MEN 2A),
multiple endocrine neoplasia type 2B (MEN 2B), neurofibromatosis, or von
Hippel-Lindau disease

- History of adrenal disease or tumor

- Hypertension with systolic blood pressure (BP) ≥160 mm Hg or diastolic BP ≥100 despite
treatment

- Untreated or inadequately treated mental illness (indicators would include symptoms
such as psychosis, hallucinations, mania, and any psychiatric hospitalization in the
last year), or treatment with anti-psychotic medications that are known to affect
glucose regulation.

- Electrically powered implants (e.g. cochlear implants, neurostimulators) that might be
susceptible to radio-frequency (RF) interference

- Unable to completely avoid acetaminophen for duration of study

- History of adverse reaction to glucagon (including allergy) besides nausea and
vomiting

- Established history of allergy or severe reaction to adhesive or tape that must be
used in the study

- History of eating disorder such as anorexia, bulimia, or diabulemia or omission of
insulin to manipulate weight

- History of intentional, inappropriate administration of insulin leading to severe
hypoglycemia requiring treatment

- Use oral [e.g. thiazolidinediones, biguanides, sulfonylureas, glitinides, dipeptidyl
peptidase-4 (DPP-4) inhibitors, sodium-glucose co-transporter 2 (SGLT-2) inhibitors]
anti-diabetic medications

- Lives in or frequents areas with poor Verizon wireless network coverage (which would
prevent remote monitoring)

- Any factors that, in the opinion of the site principal investigator or overall
principal investigator, would interfere with the safe completion of the study