Processed Orange and the Glycemic Response

The study design is a randomized, placebo controlled, double-blind, crossover. This trial
will include 33 subjects randomized to receive products A, B or C [240 mL (255 g)]. Subjects
will be randomly assigned to one of 6 sequences of 3 interventions. After the initial
screening visit, subjects will visit the Clinical and Translational Research Center (CTRC)
the Tufts Translational and Clinical Science Institute (CTSI) on three separate occasions.
Following each intervention day there will be a two week wash out period.

Inclusion Criteria:

- Males (due to potential hormonal fluctuations in female subjects) aged 30-65 y

- BMI: 25-29.9 kg/m2

- Not diabetic [diagnosed or fasting glucose >7 mmol/L (126 mg/dL)] or suffer from
other endocrine disorders

- Not having suffered a myocardial infarction/stroke in the past 12 mo

- Not suffering from renal or bowel disease or have a history of choleostatic liver or
pancreatitis

- Not on drug treatment for hyperlipidaemia, hypertension, inflammation or
hypercoagulation

- No history of alcohol misuse

- Not planning or on a weight reducing regime

- Not taking any fish oil, fatty acid or vitamin and mineral supplements

- Non smokers

Exclusion Criteria:

- Females

- Use of medications known to affect lipid metabolism, i.e., hypolipidemic or
cholesterol-lowering agents (e.g., Pravastatin, Simuvustatin)

- Use of (>2x/wk) medication for inflammation or hypercoagulation

- Anticoagulants (Warfarin)

- Inflammation - NSAID's (Tiaprofenic acid, Sulindac, Ibuprofen), Corticosteroids
(Betamethasone)

- Regular use (>2x/wk) of any acid-lowering medications, laxatives or anti-diarrheal
medications (prescription or over-the-counter [OTC])

- Use of medications known or suspected to influence blood pressure, including
beta-adrenergic blocking agents (oral or ocular) (e.g., Sotalol, Bisoprolol),
beta-adrenergic drugs, calcium channel blocking agents (Amlodipine, Nicardipine),
angiotensin converting enzyme (ACE) inhibitors (Captopril, Cilazapril), angiotensin
receptor blocking agents (Valsartan), nitrates, diuretics (Chlortalidone),
venlafaxine and sibutramine, decongestants or chloroquine

- Systolic blood pressure >150 mmHg and/or diastolic blood pressure >95 mmHg

- CVD including coronary artery disease, left ventricular hypertrophy, congestive heart
failure, cerebrovascular disease, stroke, peripheral vascular disease or dysautonomia

- Gastrointestinal diseases conditions or medications influencing gastrointestinal
absorption including active peptic ulcer disease, treatment with acid-lowering drugs
or inflammatory bowel disease

- Renal or chronic kidney disease due to any condition, renovascular disease, history
of nephrolithiasis or serum creatinine >1.5 mg/dL

- Endocrine disorders including diabetes [fasting blood glucose >7 mmol/L (126 mg/dL)
or current pharmacologic treatment for diabetes], untreated thyroid disease, adrenal
disease, pheochromocytoma, parathyroid disease or hyperuricemia

- Rheumatologic diseases including gout or inflammatory arthritis

- Active treatment for cancer of any type (except basal cell carcinoma)<1 y

- Regular use of oral steroids except topical OTC steroids

- Regular use of any dietary supplements containing vitamins, minerals, herbal or other
plant-based preparations, fish oil supplements (including cod liver oil) or
homeopathic remedies. However, subjects who are willing to refrain from the use of
these supplements for 1 mo prior to their initial visit (Visit 3) may be considered
eligible.

- Usual daily ethanol intake of>2 drinks (24 oz beer, 8 oz wine, 2 oz hard liquor)

- Cigarette smoking and/or nicotine replacement use. However, subjects who have stopped
using these products for 1 y prior to their initial visit (Visit 1) may be considered
eligible.

- Illicit drug use

- Infrequent (<3/wk) or excessive (>3/d) number of regular bowel movements

- Specific laboratory blood or urine analysis parameters of:

- Creatinine > 1.5 mg/dL

- Electrolytes, calcium, phosphorous - out of normal ranges

- ALT and AST >1.5 nmol

- Total bilirubin - above normal range

- Triglycerides ≥300 mg/dL

- Fasting glucose ≥126 mg/dL

- CBC: HCT outside of normal NEL reference ranges at the discretion of the study
physician

- WBC, PLT - outside of normal NEL reference ranges Strict vegetarians

- Those on or planning a weight reducing regime

- Unable to consume study meals or products

- Subjects with larger than 5 kg weight loss in the last 3 months.